| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,075,214 | C→T | 49.1% | N513N (AAC→AAT) | flu → | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,075,214 | 0 | C | T | 49.1% | ‑0.7 / 80.4 | 55 | N513N (AAC→AAT) | flu | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Reads supporting (aligned to +/- strand): ref base C (18/10); new base T (15/12); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01 | |||||||||||
GCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTA > W3110S.gb/2075147‑2075281 | gCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCtg < 1:427158/71‑1 (MQ=255) aaaaTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAg > 1:1095331/1‑71 (MQ=255) aaaTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAgg < 1:1369463/71‑1 (MQ=255) aTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTTATTGAAGGCa < 1:1111441/71‑1 (MQ=255) aGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAAc > 1:2070739/1‑55 (MQ=255) aGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAAc > 1:1661176/1‑55 (MQ=255) ggAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgct < 1:1639440/71‑1 (MQ=255) aaGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGATAGCCGTCAATCtg < 1:1307236/55‑1 (MQ=255) cacaCTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCAcgctgacgctga < 1:1370797/71‑1 (MQ=255) ctcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCac > 1:2795258/1‑56 (MQ=255) ctcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGa > 1:1532590/1‑71 (MQ=255) ctcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGa < 1:1698548/71‑1 (MQ=255) actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGAc < 1:1542461/69‑1 (MQ=255) actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGAc < 1:399807/69‑1 (MQ=255) ctGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGt < 1:2526082/71‑1 (MQ=255) gTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTAc > 1:1326684/1‑71 (MQ=255) gTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTAc > 1:2164387/1‑71 (MQ=255) gTCAGCAACACCACACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTAc > 1:1173332/1‑71 (MQ=255) gTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGaa > 1:265525/1‑62 (MQ=255) tCAGCAACACCACACTCACCCAGAAAGCCGTCAATCtgaatga < 1:438311/43‑1 (MQ=255) tCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTAcc > 1:232388/1‑71 (MQ=255) tCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTAcc > 1:641544/1‑71 (MQ=255) tCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTAcc > 1:93359/1‑71 (MQ=255) tCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTAcc > 1:571291/1‑71 (MQ=255) aaCACCACACTCACCCAGAAAGCCGTCAATCtgaa > 1:2796137/1‑35 (MQ=255) caccacACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacca < 1:488993/71‑1 (MQ=255) caccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGAc < 1:1159278/58‑1 (MQ=255) caccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGAc < 1:2132204/58‑1 (MQ=255) accacACTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcaccac > 1:635010/1‑71 (MQ=255) accacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgc > 1:2121396/1‑43 (MQ=255) ccacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgct < 1:822144/43‑1 (MQ=255) acacTCACCCAGAAAGCCGTCTACCTGAATGAAGGCACGCTGACGCTGAACGACAg > 1:329705/1‑56 (MQ=255) acacTCACCCAGAAAGCCGTCAATCTGAATGAAGGCacgctga > 1:1644735/1‑43 (MQ=255) acacTCACCCAGAAAGCCGTCAATCTGAATGAAGGCacgctga > 1:507857/1‑43 (MQ=255) acTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGt < 1:986349/55‑1 (MQ=255) cTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt < 1:318193/71‑1 (MQ=255) cTCACCCAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt < 1:1454559/71‑1 (MQ=255) cTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt < 1:2203093/71‑1 (MQ=255) tCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt > 1:2134503/1‑70 (MQ=255) cACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctga > 1:2319800/1‑38 (MQ=255) cccAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt < 1:1237/67‑1 (MQ=255) cAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCa > 1:2513252/1‑67 (MQ=255) cAGAAAGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCa > 1:2123894/1‑67 (MQ=255) aaGCCGTCAATCTGAATGAAGGCACGCTGACGCTGAAc > 1:1798517/1‑38 (MQ=255) gCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCa < 1:2490960/61‑1 (MQ=255) cGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCAtt > 1:968488/1‑61 (MQ=255) cGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCAtt > 1:2225004/1‑61 (MQ=255) cGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCAtt > 1:1966344/1‑61 (MQ=255) gTCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCgg < 1:1026636/71‑1 (MQ=255) tCAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCgg > 1:1889122/1‑70 (MQ=255) cAATCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa > 1:1952054/1‑71 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacca > 1:1589011/1‑46 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacca > 1:984545/1‑46 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa > 1:1558340/1‑50 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa > 1:124614/1‑50 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa > 1:2291663/1‑50 (MQ=255) aaCCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa > 1:2499160/1‑50 (MQ=255) ccTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt < 1:1254972/51‑1 (MQ=255) | GCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTA > W3110S.gb/2075147‑2075281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |