| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 330,966 | T→G | 68.3% | intergenic (+246/‑629) | betT → / → yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 330,966 | 0 | T | G | 68.3% | 91.5 / 24.5 | 44 | intergenic (+246/‑629) | betT/yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (6/8); new base G (13/17); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 | |||||||||||
AGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTG > W3110S.gb/330903‑331032 | aGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAggt > 1:2301714/1‑66 (MQ=255) tttAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCg > 1:756400/1‑70 (MQ=255) ttAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGt > 1:1764117/1‑70 (MQ=255) tAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTa < 1:1944454/70‑1 (MQ=255) aGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaa < 1:2600578/70‑1 (MQ=255) ttATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATc > 1:1634585/1‑62 (MQ=255) aTTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAAc > 1:2708560/1‑70 (MQ=255) aTTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATc < 1:1800089/67‑1 (MQ=255) tttAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATc < 1:1500617/66‑1 (MQ=255) tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTaaa > 1:2621362/1‑62 (MQ=255) tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGa < 1:2399607/70‑1 (MQ=255) tGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc < 1:1024384/61‑1 (MQ=255) tGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:717959/71‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc > 1:840056/1‑59 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTa < 1:2504859/70‑1 (MQ=255) tatTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:1179254/65‑1 (MQ=255) atTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCaa > 1:2756241/1‑71 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAAggtg < 1:2746024/36‑1 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:800914/57‑1 (MQ=255) tCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt < 1:254696/71‑1 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt > 1:2314381/1‑70 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCaa > 1:633187/1‑47 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGaaa > 1:2345187/1‑53 (MQ=255) tttGAAATGACGCATTATTCATGAGAAATGTGTATCGTa < 1:327490/39‑1 (MQ=255) aaaTGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTaa < 1:424798/71‑1 (MQ=255) aaaTGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTaa < 1:408102/71‑1 (MQ=255) aaTGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg < 1:1691304/71‑1 (MQ=255) aCGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:605403/71‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGtt < 1:1906227/55‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGtt < 1:2661663/55‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAt < 1:279496/57‑1 (MQ=255) ttattCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:956653/66‑1 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAt > 1:1339251/1‑55 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt < 1:2410610/52‑1 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGTTTTAATTATc > 1:1906556/1‑71 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTaa > 1:206885/1‑66 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTaa > 1:2074421/1‑66 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:1851106/1‑71 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:475461/1‑71 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:839394/1‑71 (MQ=255) cATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtg < 1:1846907/70‑1 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtg < 1:2360634/71‑1 (MQ=255) aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtg < 1:808680/71‑1 (MQ=255) agaAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgt > 1:991285/1‑71 (MQ=255) agaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgt > 1:456765/1‑71 (MQ=255) gaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgtg < 1:2620755/71‑1 (MQ=255) tgtgTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:2657572/52‑1 (MQ=255) | AGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTG > W3110S.gb/330903‑331032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |