| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,077,011 | G→A | 44.1% | L32L (CTG→CTA) | yeeR → | predicted membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,077,011 | 0 | G | A | 44.1% | 11.1 / 41.2 | 34 | L32L (CTG→CTA) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (12/7); new base A (6/9); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
ATCGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAA > W3110S.gb/2076949‑2077078 | aTCGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGc < 1:2172341/70‑1 (MQ=255) cGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCa > 1:1866310/1‑71 (MQ=255) cGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCa > 1:960688/1‑71 (MQ=255) gCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCat < 1:150072/71‑1 (MQ=255) gCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCa < 1:1717519/70‑1 (MQ=255) gCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCa < 1:2551145/70‑1 (MQ=255) aTTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCt > 1:1229010/1‑71 (MQ=255) ttcttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCa < 1:873040/57‑1 (MQ=255) ttcttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCa < 1:535785/57‑1 (MQ=255) cttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGtt > 1:2131527/1‑71 (MQ=255) cttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGtt > 1:2671419/1‑71 (MQ=255) cttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCatat > 1:2423882/1‑58 (MQ=255) cttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGtt > 1:1476629/1‑71 (MQ=255) cttcGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGtt > 1:1479955/1‑71 (MQ=255) cttcGCATTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGtt > 1:359808/1‑71 (MQ=255) ccTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggcc > 1:1288385/1‑71 (MQ=255) ccTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCa < 1:641756/50‑1 (MQ=255) ttGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCa < 1:1153338/46‑1 (MQ=255) ttGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTcggctcg < 1:2286972/69‑1 (MQ=255) ttCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCtt < 1:176347/71‑1 (MQ=255) ttCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCt < 1:343156/70‑1 (MQ=255) tCAGAGCATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCt > 1:1247215/1‑69 (MQ=255) cATTAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTActggct > 1:2567181/1‑71 (MQ=255) ttAATCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTATGTCTGTTCGGCCCGGCCtt < 1:1625017/62‑1 (MQ=255) aaTCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACtggctggc > 1:2368173/1‑70 (MQ=255) aaTCAGCACGGCTTCTGCGCTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACtggctggc < 1:939146/70‑1 (MQ=255) tCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGt < 1:1684179/45‑1 (MQ=255) aGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTc > 1:2030000/1‑45 (MQ=255) aGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTatat > 1:1110232/1‑71 (MQ=255) cGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATc < 1:2313548/68‑1 (MQ=255) ggCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCAcc > 1:1677348/1‑70 (MQ=255) tGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGc > 1:2495441/1‑70 (MQ=255) tGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCa > 1:1318128/1‑71 (MQ=255) gcTAGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATaa < 1:1512382/71‑1 (MQ=255) | ATCGCAGGATTTGCCATTCTTCGCCTTTTGTTCAGAGCATTAATCAGCACGGCTTCTGCGCTGGCAGGGCTCATATTGCTGTGTCTGTTCGGCCCGGCCTTACTGGCTGGCTATATCACCGAACGCATAA > W3110S.gb/2076949‑2077078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |