| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,081,459 | A→G | 10.8% | F36L (TTT→CTT) | yeeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,081,459 | 0 | A | G | 10.8% | 115.2 / 6.5 | 46 | F36L (TTT→CTT) | yeeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/19); new base G (2/3); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCG > W3110S.gb/2081393‑2081526 | ggATCTTTTTCTCAAGGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:946575/71‑1 (MQ=255)ggATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:1552593/71‑1 (MQ=255) cGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCt > 1:113365/1‑66 (MQ=255) tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATg < 1:1365587/70‑1 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACaaa > 1:1218387/1‑48 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAg > 1:390899/1‑71 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAg > 1:1371699/1‑71 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGaa > 1:1101775/1‑66 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGaa > 1:1125491/1‑66 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:455027/1‑54 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:614500/1‑54 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2753309/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1839158/1‑71 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCa > 1:1483735/1‑46 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGaa > 1:2516577/1‑63 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:1362338/1‑70 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:2064649/1‑70 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg < 1:2130154/70‑1 (MQ=255) gTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGtt < 1:1721911/71‑1 (MQ=255) gTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGtt < 1:2280990/71‑1 (MQ=255) cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt > 1:1060782/1‑66 (MQ=255) cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt > 1:961926/1‑66 (MQ=255) cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTcc < 1:375110/70‑1 (MQ=255) tttGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAt < 1:2536343/69‑1 (MQ=255) tGTTCTTTCGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2597373/70‑1 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1961768/1‑53 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2534065/1‑53 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:2218438/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1290238/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:945015/70‑1 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:354538/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2143089/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtat < 1:475842/71‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2372379/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2749419/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1630043/70‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:714240/70‑1 (MQ=255) acgacgGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATAcc < 1:164557/69‑1 (MQ=255) acgacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:859970/63‑1 (MQ=255) cgacgGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:114893/36‑1 (MQ=255) cgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGAt < 1:2762404/71‑1 (MQ=255) gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTg < 1:1349083/38‑1 (MQ=255) gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:743338/52‑1 (MQ=255) cGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1143609/1‑52 (MQ=255) cGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgtta > 1:2310826/1‑67 (MQ=255) aaCAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGagag > 1:576937/1‑70 (MQ=255) cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCAATTTTTATACCCCTGATAATGTGAGAGTCg > 1:583477/1‑71 (MQ=255) aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:563537/1‑45 (MQ=255) aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:560411/1‑45 (MQ=255) aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1488328/1‑45 (MQ=255) | GGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCG > W3110S.gb/2081393‑2081526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |