| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,096,084 | G→A | 87.5% | E160E (GAG→GAA) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,096,084 | 0 | G | A | 87.5% | 96.1 / 7.6 | 40 | E160E (GAG→GAA) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (15/20); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
AAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGA > W3110S.gb/2096020‑2096146 | aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACaa < 1:2400736/68‑1 (MQ=255)aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACaa < 1:2267174/68‑1 (MQ=255) aaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:2435853/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:371920/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:1943881/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:922849/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:1300120/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:137487/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:455896/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:2242865/1‑71 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt < 1:1457633/71‑1 (MQ=255) gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTAc > 1:2449023/1‑71 (MQ=255) cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa < 1:2691086/71‑1 (MQ=255) cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa > 1:746349/1‑71 (MQ=255) tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACt < 1:1030340/44‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt < 1:2410929/69‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAg < 1:612720/71‑1 (MQ=255) gACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAg < 1:191845/70‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAcc < 1:2102479/54‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAcc < 1:2504839/54‑1 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTa > 1:1764112/1‑57 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:441692/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:1875806/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:2315646/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:321752/71‑1 (MQ=255) aaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt < 1:1232486/49‑1 (MQ=255) aaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa < 1:1664182/71‑1 (MQ=255) cccTGAACTGGCCAATGATTGGCGAACAACTCACCAGACg > 1:986932/1‑40 (MQ=255) cccTGAACTGGCCAATGATTGGCGAACAACTCAACAGACg > 1:1354202/1‑40 (MQ=255) aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:1811105/71‑1 (MQ=255) aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:124619/71‑1 (MQ=255) aCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGATACGCTCACGTAGTGCGTaa > 1:1420965/1‑61 (MQ=255) gcAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg < 1:411760/45‑1 (MQ=255) ccAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa < 1:469867/71‑1 (MQ=255) aTGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa < 1:1398210/58‑1 (MQ=255) tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa > 1:421952/1‑52 (MQ=255) tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa > 1:57856/1‑52 (MQ=255) tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:672653/71‑1 (MQ=255) tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:897763/1‑71 (MQ=255) aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2424521/1‑71 (MQ=255) | AAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGA > W3110S.gb/2096020‑2096146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |