Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,117,348 C→T 18.2% G229D (GGC→GAC)  wcaM ← predicted colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,117,3480CT18.2% 76.1 / 9.8 33G229D (GGC→GAC) wcaMpredicted colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (16/11);  new base T (2/4);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACA  >  W3110S.gb/2117279‑2117404
                                                                     |                                                        
ttCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGcc                                                         <  1:1161102/71‑1 (MQ=255)
ttCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGcc                                                         <  1:1623919/71‑1 (MQ=255)
       cGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   >  1:1681233/1‑70 (MQ=255)
       cGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   >  1:1684347/1‑70 (MQ=255)
           aaaGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGc                                              >  1:2481218/1‑71 (MQ=255)
            aaGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   <  1:2245147/65‑1 (MQ=255)
            aaGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   <  1:501348/65‑1 (MQ=255)
             aGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTa                                                       >  1:449393/1‑60 (MQ=255)
                     aCTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGa                                     <  1:1227002/70‑1 (MQ=255)
                     aCTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGAt                                    <  1:1838908/71‑1 (MQ=255)
                        gacTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGcc                                                   <  1:2204898/51‑1 (MQ=255)
                           tGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   <  1:2178533/50‑1 (MQ=255)
                           tGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGa                                                 >  1:2404512/1‑52 (MQ=255)
                           tGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGa                                                 >  1:2301528/1‑52 (MQ=255)
                              tCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGtt                           <  1:1512668/71‑1 (MQ=255)
                              tCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGcc                                                   <  1:2593384/47‑1 (MQ=255)
                              tCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGtt                           <  1:865590/71‑1 (MQ=255)
                               cTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGTCTa                                                       >  1:2061180/1‑42 (MQ=255)
                                  ccGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGa                                                 >  1:2156318/1‑45 (MQ=255)
                                  ccGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGa                                                 >  1:9735/1‑45 (MQ=255)
                                   cGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTAc                      <  1:2556956/71‑1 (MQ=255)
                                       tAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGtt                  >  1:1705138/1‑71 (MQ=255)
                                       tAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGt                   >  1:776787/1‑70 (MQ=255)
                                         gttgttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGtt                  <  1:1581784/69‑1 (MQ=255)
                                           tgttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATg              >  1:1207607/1‑71 (MQ=255)
                                            gttATCATAAGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATgc             >  1:779061/1‑71 (MQ=255)
                                            gttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGt                            >  1:1628778/1‑56 (MQ=255)
                                            gttATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGa                >  1:315008/1‑68 (MQ=255)
                                               aTCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGa                >  1:2067102/1‑65 (MQ=255)
                                                     aGTGCTTCCCGCAAGGTCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGa    <  1:1762753/71‑1 (MQ=255)
                                                     aGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATg     >  1:785616/1‑70 (MQ=255)
                                                       tGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACa  <  1:302877/71‑1 (MQ=255)
                                                        gCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGa       >  1:2334856/1‑65 (MQ=255)
                                                          ttCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACa  <  1:711942/68‑1 (MQ=255)
                                                                  aGGTCTATGCCGATGCCCCAGTTGATTTTGCCGtt                           <  1:2219352/35‑1 (MQ=255)
                                                                     |                                                        
TTCGCCACGACAAAGTTTTTCACTGCCTGGTCTTCCGGGTAGTTGTTATCATAAGTGCTTCCCGCAAGGCCTATGCCGATGCCCCAGTTGATTTTGCCGTTGGTACAGTTGATGCGCTCGATGACA  >  W3110S.gb/2117279‑2117404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: