Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,144,746	C→T	18.4%	V114I (GTC→ATC)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,144,746	0	C	T	18.4%	112.8 / 18.5	49	V114I (GTC→ATC)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand):  ref base C (19/21);  new base T (4/5);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAG  >  W3110S.gb/2144680‑2144812                                                                   |                                                                   tGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGATcttc                                                                <  1:434155/71‑1 (MQ=255) tGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACcttc                                                                <  1:1270650/71‑1 (MQ=255)   gagaAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGATcttctt                                                              >  1:516024/1‑71 (MQ=255)    agaAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCttcttc                                                             <  1:58156/71‑1 (MQ=255)        gttaagttCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACcttc                                                                <  1:313375/64‑1 (MQ=255)               tCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGATCTTCTTCGGCTCAACCGt                                                  >  1:609996/1‑71 (MQ=255)                cGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGt                                                  >  1:1528252/1‑70 (MQ=255)                cGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGt                                                  <  1:1928777/70‑1 (MQ=255)                        aaaCGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTc                                                        <  1:1970437/56‑1 (MQ=255)                           cgcgCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttcttt                                      <  1:2328109/71‑1 (MQ=255)                            gcgcATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttcttt                                      <  1:2315916/70‑1 (MQ=255)                              gcATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa                                    <  1:98061/70‑1 (MQ=255)                                 tCCGTCAGCAACAAAATGCCTTCGAGAATGATTACCTTCTTCGGCTCAACCGTCACCGtttcttt                                      <  1:611236/65‑1 (MQ=255)                                 tCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttcttt                                      >  1:63219/1‑65 (MQ=255)                                 tCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttcttt                                      <  1:1999963/65‑1 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGATCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg                              <  1:810091/71‑1 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAAc                                                     >  1:1097444/1‑48 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAAc                                                     >  1:399668/1‑48 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGt                                                  >  1:2209980/1‑51 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa                                    >  1:526717/1‑65 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg                              <  1:1804692/71‑1 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg                              >  1:59044/1‑71 (MQ=255)                                   cGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg                              >  1:1600533/1‑71 (MQ=255)                                     tCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTa                            <  1:156404/71‑1 (MQ=255)                                     tCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTa                            <  1:78803/71‑1 (MQ=255)                                     tCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCGCAACCGTCACCGTTTCTTTCATACGCGTa                            <  1:305573/71‑1 (MQ=255)                                           acaaAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCAcc                                              <  1:118751/47‑1 (MQ=255)                                           acaaAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCa                      <  1:599927/71‑1 (MQ=255)                                            caaAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTGTTTCATACGCGTATGTTCaa                     <  1:199955/71‑1 (MQ=255)                                            caaAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCaa                     <  1:1464985/71‑1 (MQ=255)                                            caaAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCaa                     <  1:165319/71‑1 (MQ=255)                                             aaaaTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCaa                     >  1:1473256/1‑70 (MQ=255)                                             aaaaTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCaa                     >  1:603075/1‑70 (MQ=255)                                              aaaTGCCTTCGAGAATGATGATCTTCTTCGGCTCAACCGTCAcc                                              <  1:865664/44‑1 (MQ=255)                                               aaTGCCTTCGAGAATGATGATCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACAt                  <  1:1317863/71‑1 (MQ=255)                                                aTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAc                                 >  1:1772520/1‑55 (MQ=255)                                                aTGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACa                   >  1:689183/1‑69 (MQ=255)                                                            atgatgTCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACa                   >  1:358899/1‑57 (MQ=255)                                                            atgatgATCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCgg     <  1:674114/71‑1 (MQ=255)                                                            atgatgACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTa                            >  1:1699208/1‑48 (MQ=255)                                                            atgatgACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACa                   >  1:733245/1‑57 (MQ=255)                                                             tgatgaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCTTATGTTCAACATAGCTGTAAACCgg     >  1:242303/1‑70 (MQ=255)                                                             tgatgaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGATGTAAACCgg     >  1:1576062/1‑70 (MQ=255)                                                               atgaTCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCgg     >  1:2334985/1‑68 (MQ=255)                                                               atgaTCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCgg     >  1:646043/1‑68 (MQ=255)                                                               atgaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCaa                     <  1:72426/52‑1 (MQ=255)                                                               atgaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAg  >  1:1494581/1‑71 (MQ=255)                                                               atgaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAg  >  1:36272/1‑71 (MQ=255)                                                                 gaCCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTaa          <  1:1402243/61‑1 (MQ=255)                                                                   |                                                                   TGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAG  >  W3110S.gb/2144680‑2144812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑