Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,213,335	A→G	10.6%	N301S (AAT→AGT)	yehQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,213,335	0	A	G	10.6%	118.5 / 7.3	47	N301S (AAT→AGT)	yehQ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/20);  new base G (2/3);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

AGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACG  >  W3110S.gb/2213277‑2213399                                                           |                                                                 agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga                                                      <  1:2495170/71‑1 (MQ=255) agccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga                                                      <  1:1517250/71‑1 (MQ=255)  gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAaga                                                      <  1:2723715/70‑1 (MQ=255)  gccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga                                                      <  1:2235232/70‑1 (MQ=255)    caCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAgaga                                                    >  1:550993/1‑70 (MQ=255)     aCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATg                                                  >  1:1604111/1‑71 (MQ=255)      cTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa                                                                  <  1:145308/54‑1 (MQ=255)      cTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa                                                                  <  1:317383/54‑1 (MQ=255)       tataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTaaa                                                                  <  1:842002/53‑1 (MQ=255)        ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGcc                                               >  1:1770279/1‑71 (MQ=255)        ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAtaga                                                    >  1:1283272/1‑66 (MQ=255)        ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAgaga                                                    >  1:1147149/1‑66 (MQ=255)        ataATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGcc                                               >  1:271907/1‑71 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCTCACAAGAGATGGc                                                <  1:1752240/69‑1 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAaga                                                      <  1:2369883/63‑1 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAgaga                                                    >  1:345281/1‑65 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc                                              >  1:2080124/1‑71 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc                                              >  1:536173/1‑71 (MQ=255)         taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGccc                                              >  1:2344778/1‑71 (MQ=255)                   gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCTACAg                                    <  1:233170/71‑1 (MQ=255)                   gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAg                                    <  1:1480885/71‑1 (MQ=255)                    aaTGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAagag                                                     <  1:1460065/53‑1 (MQ=255)                        cTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTAtt                                >  1:692606/1‑70 (MQ=255)                              tCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAg                         <  1:456034/71‑1 (MQ=255)                                aGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATgg                                                 >  1:1334991/1‑45 (MQ=255)                                aGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGt                        <  1:1957928/70‑1 (MQ=255)                                 gCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGa                           <  1:1050193/66‑1 (MQ=255)                                 gCTTGCGGCATTAAACAGTCGATTAAGTTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGa                           <  1:1570904/66‑1 (MQ=255)                                  cTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcc                     <  1:2284120/71‑1 (MQ=255)                                    tGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTa                                  >  1:1248655/1‑56 (MQ=255)                                         cATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCtgtg              >  1:1045108/1‑71 (MQ=255)                                           ttAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTg                            <  1:2778818/55‑1 (MQ=255)                                           ttAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcctc                   <  1:559828/64‑1 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                      >  1:2760149/1‑59 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                      >  1:555111/1‑59 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt          >  1:2697716/1‑71 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt          >  1:2537495/1‑71 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt          >  1:825042/1‑71 (MQ=255)                                             aaaCAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt          >  1:1298802/1‑71 (MQ=255)                                                   tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa                          <  1:1697993/49‑1 (MQ=255)                                                   tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa                          <  1:1803719/49‑1 (MQ=255)                                                   tCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGaa                          <  1:1376090/49‑1 (MQ=255)                                                    cGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCAc   >  1:1294076/1‑71 (MQ=255)                                                     gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcctc                   >  1:2179288/1‑54 (MQ=255)                                                     gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg  >  1:1692512/1‑71 (MQ=255)                                                     gATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg  <  1:2271195/71‑1 (MQ=255)                                                      aTTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGtt                       <  1:396685/49‑1 (MQ=255)                                                      aTTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGc             <  1:502860/59‑1 (MQ=255)                                                       ttAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACg  <  1:2176476/69‑1 (MQ=255)                                                        tAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                      >  1:2372672/1‑48 (MQ=255)                                                           |                                                                 AGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACG  >  W3110S.gb/2213277‑2213399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑