| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,215,186 | A→G | 69.0% | V115V (GTT→GTC) | yehS ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,215,186 | 0 | A | G | 69.0% | 37.9 / 20.8 | 29 | V115V (GTT→GTC) | yehS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base G (10/10); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGC > W3110S.gb/2215123‑2215250 | gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:1574317/70‑1 (MQ=255) ttCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGAc > 1:1955770/1‑60 (MQ=255) cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGaa > 1:2191521/1‑64 (MQ=255) gTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:163591/55‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg < 1:1247567/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:271038/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:2675579/71‑1 (MQ=255) tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:2588662/71‑1 (MQ=255) cGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGa < 1:2309272/70‑1 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGAt < 1:91455/52‑1 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGAt < 1:1143553/52‑1 (MQ=255) atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAg > 1:559146/1‑52 (MQ=255) atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAg > 1:2575529/1‑52 (MQ=255) atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:1927283/1‑71 (MQ=255) atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:1105059/1‑71 (MQ=255) atcGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:599776/1‑71 (MQ=255) gCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAg > 1:895998/1‑71 (MQ=255) gCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAg > 1:2093344/1‑71 (MQ=255) gCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAg > 1:2703987/1‑71 (MQ=255) gCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTGTTCAg > 1:2179112/1‑71 (MQ=255) tAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:2638125/71‑1 (MQ=255) tAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGaa < 1:459154/71‑1 (MQ=255) tttCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGa < 1:812888/39‑1 (MQ=255) tCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATCTCATCGGTTTTCAGCGAAAAcg > 1:1838497/1‑70 (MQ=255) ccGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:2637685/70‑1 (MQ=255) ccGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:1268902/70‑1 (MQ=255) aaTCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGc > 1:1478497/2‑71 (MQ=255) tCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:1681225/64‑1 (MQ=255) gAAACTCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGa > 1:2401636/1‑56 (MQ=255) | GCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGC > W3110S.gb/2215123‑2215250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |