Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,155,146:1 +A 100% coding (783/870 nt) prkB ← predicted phosphoribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,155,1461.A83.3% 39.3 / 5.5 18coding (783/870 nt)prkBpredicted phosphoribulokinase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base A (15/0);  total (15/3)
Fisher's exact test for biased strand distribution p-value = 1.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAG  >  W3110S.gb/4155108‑4155200
                                       |                                                      
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:1324360/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:754469/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:603621/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:425583/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:1573010/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:1616036/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:343051/1‑71 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑aa                                                      >  1:296705/1‑40 (MQ=255)
attaattCCATTGCCAGACCCATTTTGCCGCCAGGTACCAACTAACGTATTGATGTGGGAaatgaa                              >  1:171842/1‑66 (MQ=255)
attaattCCATCGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc                         >  1:1869653/1‑71 (MQ=255)
 ttaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCt                       <  1:1027713/71‑1 (MQ=255)
 ttaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCt                       <  1:933844/71‑1 (MQ=255)
 ttaattCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCt                       <  1:942843/71‑1 (MQ=255)
           tGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTAtt                                             >  1:307336/1‑40 (MQ=38)
           tGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGAtgt                                        >  1:1557329/1‑45 (MQ=255)
           tGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc              >  1:400999/1‑71 (MQ=255)
           tGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc              >  1:1439271/1‑71 (MQ=255)
           tGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc              >  1:776284/1‑71 (MQ=255)
                        ttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAg  >  1:1454381/1‑70 (MQ=255)
                                       |                                                      
ATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAG  >  W3110S.gb/4155108‑4155200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑