| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,111,828:1 | +C | 100% | coding (917/1536 nt) | mdoG → | glucan biosynthesis protein, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,111,828 | 1 | . | C | 75.0% | 14.9 / 1.6 | 8 | coding (917/1536 nt) | mdoG | glucan biosynthesis protein, periplasmic |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (6/0); total (6/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
AATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAA‑CCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATG > W3110S.gb/1111758‑1111890 | aaTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa > 1:1156066/1‑71 (MQ=255)aaTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa > 1:677358/1‑71 (MQ=255)aaTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa > 1:660971/1‑71 (MQ=255)aaTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa > 1:1614999/1‑71 (MQ=255) aTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa < 1:118011/70‑1 (MQ=255) aTGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGaaaa < 1:1587185/70‑1 (MQ=255) ggCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAA‑ccc > 1:1512320/1‑71 (MQ=255) ggCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAA‑ccc > 1:393033/1‑71 (MQ=255) ggCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAA‑cc > 1:974721/1‑70 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgcg > 1:1478/1‑71 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgcg > 1:1454809/1‑71 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgcg > 1:1379599/1‑71 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgcg > 1:1251727/1‑71 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgcg > 1:734700/1‑71 (MQ=255) aTAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCCGCAAGGCTTTGGTCTGTTGCAgc > 1:748839/1‑70 (MQ=255) cATGGAAAA‑CCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCgatg < 1:438061/71‑1 (MQ=255) cATGGAAAA‑CCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCgatg < 1:1246765/71‑1 (MQ=255) | AATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAA‑CCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATG > W3110S.gb/1111758‑1111890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |