| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,777,419:1 | +A | 100% | coding (3964/4581 nt) | ypjA ← | adhesin‑like autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,777,419 | 1 | . | A | 77.3% | 102.0 / 32.9 | 44 | coding (3964/4581 nt) | ypjA | adhesin‑like autotransporter |
| Reads supporting (aligned to +/- strand): ref base . (1/9); new base A (20/14); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAG > W3110S.gb/2777357‑2777478 | aTGTTCCCAACTGGCATAGCCTCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGa < 1:450641/71‑1 (MQ=255)aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAAccta < 1:1229113/71‑3 (MQ=255)aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGa < 1:963684/71‑1 (MQ=255)aTGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGa < 1:168839/71‑1 (MQ=255) tGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTTCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGa < 1:1043617/70‑1 (MQ=255) ttCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGata > 1:1915620/1‑71 (MQ=255) cAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:66202/71‑1 (MQ=255) cAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:272980/71‑1 (MQ=255) cAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATAtgtg < 1:1625861/71‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATggg < 1:1081888/70‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATggg < 1:1223364/70‑1 (MQ=255) gCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATggg < 1:464474/70‑1 (MQ=255) cATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATggg < 1:1699274/69‑1 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAg > 1:290347/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAg > 1:194619/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAg > 1:1553976/1‑71 (MQ=255) gccgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAg > 1:1455670/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGcc > 1:562162/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGcc > 1:884524/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGcc > 1:842639/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGcc > 1:465077/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGcc > 1:1965607/1‑71 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGc > 1:1072100/1‑70 (MQ=255) cgccCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATACGTGAATGGGAATAGcc > 1:813357/1‑71 (MQ=255) tAACTGCCCACACTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAgcgc > 1:1505218/1‑71 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGTGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:910851/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:622556/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:1904602/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:816106/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:801898/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:726922/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:598733/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:569144/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:437195/71‑1 (MQ=255) cacTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:434289/71‑1 (MQ=255) acTGCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGt < 1:1381710/70‑1 (MQ=255) gCCATGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAAtt > 1:1643576/1‑71 (MQ=255) aTGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCt > 1:1530229/1‑71 (MQ=255) aTGTCCTCCGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCt > 1:1400934/1‑71 (MQ=255) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:1149217/1‑69 (MQ=255) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:1340832/1‑69 (MQ=255) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:860912/1‑69 (MQ=255) tcctccGCGATCAAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt > 1:408822/1‑69 (MQ=255) tcctccGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAg > 1:893642/1‑71 (MQ=255) | ATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATC‑AAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAG > W3110S.gb/2777357‑2777478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |