| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 234,234 | C→T | 59.5% | L183L (TTG→TTA) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 234,234 | 0 | C | T | 59.5% | 18.1 / 43.6 | 37 | L183L (TTG→TTA) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (12/3); new base T (13/9); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATT > W3110S.gb/234179‑234286 | ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaag < 1:274144/70‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:98759/70‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:70691/70‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa > 1:1878781/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:1348202/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:484667/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:431721/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:263478/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:1888794/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:1818395/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:1472/1‑70 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa > 1:1510667/1‑70 (MQ=255) cGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaag < 1:1759068/53‑1 (MQ=255) aaCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:202088/52‑1 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGt > 1:63604/1‑42 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1053084/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:748220/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:708816/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:589101/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:545845/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:493174/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:440988/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1740388/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1874392/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1790508/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGG‑ATAATACTCAAAGCAAACTTCata > 1:1959930/1‑70 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:36607/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1580683/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:1400675/1‑71 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:964273/1‑71 (MQ=255) ataataATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:274043/71‑1 (MQ=255) ataataATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:85156/71‑1 (MQ=255) ataataATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:1782863/71‑1 (MQ=255) ataataATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:1562126/71‑1 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAATGTATAtt < 1:1880421/70‑1 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:481917/70‑1 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:1738509/70‑1 (MQ=255) | TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATT > W3110S.gb/234179‑234286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |