| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,381,341 | T→C | 37.5% | S248S (AGT→AGC) | degQ → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,381,341 | 0 | T | C | 37.5% | 23.7 / 31.3 | 32 | S248S (AGT→AGC) | degQ | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base T (13/7); new base C (3/9); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
ATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGG > W3110S.gb/3381273‑3381406 | aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:813521/71‑1 (MQ=255)aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1249641/71‑1 (MQ=255)aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:724810/71‑1 (MQ=255)aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:41690/71‑1 (MQ=255)aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:27327/71‑1 (MQ=255)aTTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1906761/71‑1 (MQ=255) ttGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1667953/70‑1 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:1225445/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:84814/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:803754/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:801407/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:32903/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:315499/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:178636/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:1246538/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:1267285/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:1711913/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:1618591/1‑70 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:1696256/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCACTAATATGgcg > 1:357705/1‑69 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGaa > 1:149191/1‑69 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAaca > 1:1963393/1‑71 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:121308/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:774327/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:470541/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:354682/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:1720271/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:1938586/71‑1 (MQ=255) gcggcgGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATc < 1:176910/71‑1 (MQ=255) cATCCCCAGCAATATGGCGAGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:450109/71‑1 (MQ=255) aaTCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:232293/70‑1 (MQ=255) cccAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAgg > 1:1409554/1‑71 (MQ=255) | ATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGG > W3110S.gb/3381273‑3381406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |