| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,442,070 | A→G | 40.0% | T497A (ACG→GCG) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,442,070 | 0 | A | G | 40.0% | 33.9 / 70.9 | 60 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (29/7); new base G (12/12); total (41/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01 | |||||||||||
TCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTAT > W3110S.gb/3442008‑3442132 | tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:544257/1‑69 (MQ=255)tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:1180149/1‑69 (MQ=255)tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:1025228/1‑69 (MQ=255)tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:1244621/1‑69 (MQ=255)tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:22691/1‑69 (MQ=255)tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:1924110/1‑69 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:611812/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:1773655/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:313263/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:1293404/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:1437542/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:1315845/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:228736/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:206737/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:87303/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCa > 1:1421013/1‑67 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:1588832/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:68938/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:53560/1‑68 (MQ=255) gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:1293902/71‑1 (MQ=255) gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:1188478/71‑1 (MQ=255) tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCa < 1:1770166/71‑1 (MQ=255) gatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:546741/1‑68 (MQ=255) gatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:1084070/1‑68 (MQ=255) atTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAaag < 1:1379884/70‑1 (MQ=255) atTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAaag < 1:339620/70‑1 (MQ=255) tGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaag > 1:862249/1‑68 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:1567791/1‑70 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:1417783/1‑70 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:1408624/1‑70 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:55272/1‑70 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:874548/1‑70 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAg > 1:1252359/1‑70 (MQ=255) tGGATGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATaa > 1:1318932/1‑57 (MQ=255) gCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCa < 1:1679440/70‑1 (MQ=255) gCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCa < 1:618191/70‑1 (MQ=255) cgcgATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:205851/70‑1 (MQ=255) cgcgATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:1097609/70‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAATGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:346098/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTTGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:1791357/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:88185/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:66757/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:901281/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:521953/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:1919941/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:1898702/65‑1 (MQ=255) tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:1655363/65‑1 (MQ=255) gCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:410527/64‑1 (MQ=255) ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCAc > 1:727355/1‑71 (MQ=255) ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCAc > 1:1426734/1‑71 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:515742/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:499148/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:355117/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:200429/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:1611770/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:1488566/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:1356261/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:1336253/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:1185013/1‑69 (MQ=255) tACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCta > 1:1947230/1‑68 (MQ=255) | TCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTAT > W3110S.gb/3442008‑3442132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |