| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,447,843 | A→G | 76.9% | V1163V (GTT→GTC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,447,843 | 0 | A | G | 76.9% | 49.8 / 11.6 | 26 | V1163V (GTT→GTC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (2/18); total (5/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGAC > W3110S.gb/3447775‑3447886 | tACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:144186/71‑1 (MQ=255)tACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:1846552/71‑1 (MQ=255) ccGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:1308540/71‑1 (MQ=255) ccGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:529216/71‑1 (MQ=255) ccGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:1534695/71‑1 (MQ=255) ccGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:167243/71‑1 (MQ=255) cGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:1885094/63‑1 (MQ=255) gggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:1181442/71‑1 (MQ=255) gggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:1535859/71‑1 (MQ=255) gggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:179680/71‑1 (MQ=255) gggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:386632/71‑1 (MQ=255) tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGcc < 1:527325/71‑1 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCgg > 1:108548/1‑71 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCgg > 1:1073362/1‑71 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAg < 1:779510/62‑1 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAg < 1:484053/62‑1 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAg < 1:1328736/62‑1 (MQ=255) ccAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt < 1:50575/52‑1 (MQ=255) cAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGct > 1:1826474/1‑71 (MQ=255) cAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGct > 1:437241/1‑71 (MQ=255) cAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGct > 1:1187178/1‑71 (MQ=255) acgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCg < 1:1846456/71‑1 (MQ=255) acgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCg < 1:546277/71‑1 (MQ=255) acgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCg < 1:709820/71‑1 (MQ=255) tttACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgac < 1:1667839/71‑1 (MQ=255) tttACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgac < 1:1426793/71‑1 (MQ=255) | TACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGAC > W3110S.gb/3447775‑3447886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |