Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,567,710 T→C 100% R102R (CGT→CGC)  yihQ → alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,567,7100TC77.8% 13.4 / ‑0.0 9R102R (CGT→CGC) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (5/2);  total (7/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGC  >  W3110S.gb/3567672‑3567772
                                      |                                                              
aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACgc                                         >  1:893425/1‑61 (MQ=255)
aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTaa                                >  1:474035/1‑71 (MQ=255)
                tctcTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTgg                >  1:1471912/1‑71 (MQ=255)
                      ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg           <  1:1693107/70‑1 (MQ=255)
                      ccGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTgcg           <  1:177997/70‑1 (MQ=255)
                              cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc  >  1:1069718/1‑71 (MQ=255)
                              cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc  >  1:1393409/1‑71 (MQ=255)
                              cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGc  >  1:1852672/1‑71 (MQ=255)
                              cAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATATGGCTGCGCCTTGCCGc  >  1:1691531/1‑71 (MQ=255)
                                      |                                                              
AGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGC  >  W3110S.gb/3567672‑3567772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑