| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,587,198 | C→T | 53.5% | E840K (GAA→AAA) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,587,198 | 0 | C | T | 53.5% | 2.2 / 61.5 | 43 | E840K (GAA→AAA) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base C (18/2); new base T (13/10); total (31/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACA > W3110S.gb/3587137‑3587248 | atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGc > 1:1640231/1‑68 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:850103/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:811548/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:310835/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:221094/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:1436987/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:1962874/1‑70 (MQ=255)atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTg > 1:1162709/1‑70 (MQ=255) cGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCa > 1:536035/1‑70 (MQ=255) ccGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCacgac < 1:148154/71‑1 (MQ=255) tttGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAg < 1:1827805/71‑1 (MQ=255) tttGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAg < 1:693069/71‑1 (MQ=255) tttGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAg < 1:688824/71‑1 (MQ=255) tGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCa > 1:326186/1‑71 (MQ=255) cATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:1219720/1‑64 (MQ=255) cATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:1877540/1‑64 (MQ=255) cATTGGCGCGTTAATGGATGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:1367405/1‑64 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAg < 1:206700/71‑1 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:1745157/1‑68 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:1703753/1‑68 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:1507422/1‑68 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:468564/1‑68 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:453689/1‑68 (MQ=255) aTTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:407455/1‑68 (MQ=255) ttGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAg < 1:1901738/70‑1 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:1611827/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:599620/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:555250/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:36197/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCacgac > 1:1377432/1‑37 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGAt > 1:1289430/1‑70 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGAt > 1:1696073/1‑70 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGAt > 1:835138/1‑70 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:1494457/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:528289/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:1247248/1‑71 (MQ=255) ggCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATa > 1:1751417/1‑71 (MQ=255) cgcgTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1544441/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1920576/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1275763/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1273485/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:158396/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1756897/71‑1 (MQ=255) | ATCGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACA > W3110S.gb/3587137‑3587248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |