| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,591,880 | C→T | 56.9% | M201I (ATG→ATA) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,591,880 | 0 | C | T | 56.9% | 19.1 / 65.4 | 51 | M201I (ATG→ATA) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (10/12); new base T (13/16); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTAC > W3110S.gb/3591815‑3591947 | ggAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTgg > 1:1071239/1‑71 (MQ=255)ggAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTgg > 1:562667/1‑71 (MQ=255)ggAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTgg > 1:425508/1‑71 (MQ=255)ggAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTgg > 1:1362259/1‑71 (MQ=255) gAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGt < 1:1168856/71‑1 (MQ=255) gAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGt < 1:34870/71‑1 (MQ=255) ctAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:1154817/70‑1 (MQ=255) ctAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAAc < 1:1307404/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAat < 1:1155019/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAat < 1:248266/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAat < 1:240732/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAat < 1:1904262/70‑1 (MQ=255) aTCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAat < 1:1467849/70‑1 (MQ=255) gcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTc < 1:981434/71‑1 (MQ=255) gcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTc < 1:56786/71‑1 (MQ=255) gcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTc < 1:1292494/71‑1 (MQ=255) gcCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTc < 1:1295019/71‑1 (MQ=255) agagAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGAc > 1:564976/1‑71 (MQ=255) agagAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGAc < 1:1213425/71‑1 (MQ=255) agagAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGAc < 1:1526621/71‑1 (MQ=255) gaATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACttt > 1:1070481/1‑71 (MQ=255) gaATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACttt > 1:1845677/1‑71 (MQ=255) gaATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACttt > 1:1750200/1‑71 (MQ=255) gaATCAATGGTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACttt > 1:644209/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCt > 1:1608494/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCt > 1:177987/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCt > 1:1798420/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCt > 1:1449848/1‑71 (MQ=255) cAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCt > 1:1465102/1‑71 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:563905/71‑1 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:167295/71‑1 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:1706518/71‑1 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:1969407/71‑1 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:192332/71‑1 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCaa > 1:1459469/1‑70 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCaa > 1:1461956/1‑70 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCaa > 1:109879/1‑70 (MQ=255) gTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATATTCGACTTTGCCTTCa > 1:598248/1‑69 (MQ=255) tAAAACTTTTTGCCGTTACTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:1082944/70‑1 (MQ=255) ccGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGAc < 1:614202/71‑1 (MQ=255) ccGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGAc < 1:1575447/71‑1 (MQ=255) aCTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg > 1:1437099/1‑70 (MQ=255) aCTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg > 1:1182236/1‑70 (MQ=255) aCTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg > 1:1899146/1‑70 (MQ=255) aCTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg > 1:1343337/1‑70 (MQ=255) aCTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATg > 1:1442433/1‑70 (MQ=255) cTTTGTTTATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGa < 1:1294247/70‑1 (MQ=255) cTTTGTTTATTGCTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGa < 1:426364/70‑1 (MQ=255) tGTTTATT‑GTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAat < 1:204485/48‑1 (MQ=255) ttCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTAc < 1:1207468/70‑1 (MQ=255) ttCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTAc < 1:932124/70‑1 (MQ=255) | GGAAATTTCTTTTCTTCTAATCGCGCCAGAGAATCAATGGTAAAACTTTTTGCCGTTACTTTGTTCATTGGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTAC > W3110S.gb/3591815‑3591947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |