| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,634,018 | T→C | 22.7% | V46A (GTA→GCA) | yigF → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,634,018 | 0 | T | C | 22.7% | 39.1 / 9.2 | 22 | V46A (GTA→GCA) | yigF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (5/12); new base C (2/3); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG > W3110S.gb/3633953‑3634083 | aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:1445881/71‑1 (MQ=255) cTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAAc < 1:190650/70‑1 (MQ=255) gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAttt < 1:1252842/71‑1 (MQ=255) gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAttt < 1:1829963/71‑1 (MQ=255) cAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTc < 1:517735/71‑1 (MQ=255) aaCACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTc < 1:146197/70‑1 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1215936/1‑70 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:817563/1‑70 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:644635/1‑70 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1310395/1‑70 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1119163/1‑70 (MQ=255) tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1860471/1‑70 (MQ=255) tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:273609/1‑70 (MQ=255) aGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatt < 1:162739/71‑1 (MQ=255) aGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatt < 1:150459/71‑1 (MQ=255) aGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatt < 1:466155/71‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:1459711/61‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:771189/61‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:931995/61‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:970203/61‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCT‑‑TGTTCCTGGATATATTTATTTg < 1:1689612/71‑1 (MQ=255) ttAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTCCTTTCTTCTGTTCCTGGa < 1:1780600/61‑1 (MQ=255) | ACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG > W3110S.gb/3633953‑3634083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |