Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,682,573	G→A	62.5%	L211L (CTG→TTG)	ilvD ←	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,682,573	0	G	A	62.5%	9.1 / 12.6	16	L211L (CTG→TTG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base A (3/7);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACA  >  W3110S.gb/3682511‑3682639                                                               |                                                                   cAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCgg                                                            >  1:299020/1‑71 (MQ=255)            ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:1249723/71‑1 (MQ=255)            ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:1421612/71‑1 (MQ=255)            ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:1824628/71‑1 (MQ=255)            ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:493072/71‑1 (MQ=255)            ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:621507/71‑1 (MQ=255)             gTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:1168746/70‑1 (MQ=255)             gTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc                                                 <  1:542113/70‑1 (MQ=255)                      ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCg                                                             >  1:1642277/1‑49 (MQ=255)                      ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAg                                       >  1:396477/1‑71 (MQ=255)                      ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAg                                       >  1:798037/1‑71 (MQ=255)                                  cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATcc                           <  1:1414822/71‑1 (MQ=255)                                  cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATcc                           <  1:1417137/71‑1 (MQ=255)                                                           cccAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca  >  1:102351/1‑71 (MQ=255)                                                           cccAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca  >  1:1617256/1‑71 (MQ=255)                                                           cccAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGaca  >  1:236238/1‑71 (MQ=255)                                                               |                                                                   CAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACA  >  W3110S.gb/3682511‑3682639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑