Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,709,115	C→T	71.8%	E138E (GAG→GAA)	asnA ←	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,709,115	0	C	T	71.8%	59.0 / 27.0	39	E138E (GAG→GAA)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base T (4/24);  total (9/30)
Fisher's exact test for biased strand distribution p-value = 8.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCC  >  W3110S.gb/3709061‑3709179                                                       |                                                                 gCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTct                                                  <  1:407338/71‑2 (MQ=255) gCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCa                                                  <  1:1301759/71‑1 (MQ=255) gCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCa                                                  <  1:611777/71‑1 (MQ=255) gCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCa                                                  <  1:562892/71‑1 (MQ=255)  ccAAACTCTTCGCTAACCGCAGCTTTGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCag                                                 <  1:1665147/71‑1 (MQ=255)  ccAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCGCAGATCGCTTCTACCGTGCTTTTCag                                                 <  1:85435/71‑1 (MQ=255)  ccAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCag                                                 <  1:318015/71‑1 (MQ=255)  ccAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCag                                                 <  1:1042170/71‑1 (MQ=255)  ccAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtttt                                                    >  1:1500783/1‑68 (MQ=255)       ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTc                                             >  1:422636/1‑70 (MQ=255)       ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTc                                             >  1:1243029/1‑70 (MQ=255)       ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTc                                             >  1:1314541/1‑70 (MQ=255)       ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTc                                             >  1:184605/1‑70 (MQ=255)       ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTc                                             >  1:399358/1‑70 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:587326/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:1657889/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:420846/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:403658/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:213173/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:248185/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:983139/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:441419/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:1930206/71‑1 (MQ=255)             gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAAtt                                      <  1:672017/71‑1 (MQ=255)                                         cccGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGt                          >  1:1374817/1‑55 (MQ=255)                                         cccGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTc          >  1:1050322/1‑71 (MQ=255)                                         cccGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTc          >  1:1460772/1‑71 (MQ=255)                                                aGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTcc   <  1:1248604/71‑1 (MQ=255)                                                 gATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTccc  <  1:24742/71‑1 (MQ=255)                                                 gATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTccc  <  1:1829184/71‑1 (MQ=255)                                                 gATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:371706/64‑1 (MQ=255)                                                 gATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:1087052/64‑1 (MQ=255)                                                 gATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:960457/64‑1 (MQ=255)                                                  aTCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:192970/63‑1 (MQ=255)                                                  aTCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:1694896/63‑1 (MQ=255)                                                  aTCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:1631004/63‑1 (MQ=255)                                                  aTCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:1445541/63‑1 (MQ=255)                                                  aTCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:675472/63‑1 (MQ=255)                                                   tCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc         <  1:1317092/62‑1 (MQ=255)                                                       |                                                                 GCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCC  >  W3110S.gb/3709061‑3709179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑