| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,783,965 | C→T | 35.9% | G13D (GGC→GAC) | yidI ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,783,965 | 0 | C | T | 35.9% | 36.4 / 35.1 | 39 | G13D (GGC→GAC) | yidI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (8/6); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
CGGAGAAAGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAA > W3110S.gb/3783900‑3784027 | cGGAGAAAGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAAt > 1:276138/1‑71 (MQ=255)cGGAGAAAGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAAt > 1:606179/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt > 1:687158/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:217014/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:433806/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:1637931/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt > 1:771393/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:1401980/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt > 1:920030/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt > 1:1137250/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt > 1:962341/1‑71 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCAAATGAAc > 1:1835473/1‑52 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCTATGAACTGATTTTATTTTTAGCTAt < 1:1412836/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1023449/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:581528/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:921261/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:277854/71‑1 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1701421/71‑1 (MQ=255) ggATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1002790/70‑1 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAAGAGCATGCCCAATGAA‑‑GATTTTATTTTGAGCTATGATa > 1:635407/1‑71 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGa > 1:962324/1‑71 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGa > 1:959458/1‑71 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGa > 1:471741/1‑71 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGa > 1:304878/1‑71 (MQ=255) gATAATCCACATCATTAAAGCGATGTCACCGAACATCATGCCCAATGAACTGATTTTATTTTGAGatat < 1:878813/69‑1 (MQ=255) tAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:607724/67‑1 (MQ=255) tCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGattt < 1:382127/51‑1 (MQ=255) tCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGattt < 1:649816/51‑1 (MQ=255) tCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGattt < 1:1840696/51‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGatttta > 1:1873597/1‑51 (MQ=255) catcatTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGattt < 1:298165/47‑1 (MQ=255) atcatTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTg < 1:1253100/71‑1 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAcc > 1:1619816/1‑71 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAcc > 1:1464367/1‑71 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAcc > 1:336779/1‑71 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAc > 1:133555/1‑70 (MQ=255) cAGCATGTCCAATGAACTGATTTTATTTTGAGCTAt < 1:478095/36‑1 (MQ=255) cAGCATGTCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCaa > 1:1452568/1‑70 (MQ=255) cAGCATGTCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCaa > 1:81470/1‑70 (MQ=255) | CGGAGAAAGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAA > W3110S.gb/3783900‑3784027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |