| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,798,160 | T→C | 14.9% | M49T (ATG→ACG) | yicN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,798,160 | 0 | T | C | 14.9% | 118.6 / 13.6 | 47 | M49T (ATG→ACG) | yicN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/24); new base C (3/4); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TCAGCGATCGGCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAC > W3110S.gb/3798106‑3798227 | tcAGCGATCGGCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGt > 1:783065/1‑71 (MQ=255)tcAGCGATCGGCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGt > 1:965910/1‑71 (MQ=255)tcAGCGATCGGCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGt > 1:262441/1‑71 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:956304/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:1174954/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:795190/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:1211285/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:191117/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:1884629/71‑1 (MQ=255) gCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAAtttt < 1:1813364/71‑1 (MQ=255) gATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTAtt < 1:954744/70‑1 (MQ=255) gATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTAtt < 1:895662/70‑1 (MQ=255) gATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTAtt < 1:439105/70‑1 (MQ=255) gATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTAtt < 1:1656168/70‑1 (MQ=255) gTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCg < 1:1814094/71‑1 (MQ=255) gTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCg < 1:1839002/71‑1 (MQ=255) gTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCg < 1:1053358/71‑1 (MQ=255) gTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCg < 1:1495975/71‑1 (MQ=255) cccGTGGAGTCGATGGTCGATCAAACGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTcc > 1:1041905/1‑71 (MQ=255) cccGTGGAGTCGATGGTCGATCAAACGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTcc > 1:335549/1‑71 (MQ=255) cccGTGGAGTCGATGGTCGATCAAACGGGAAAGACAGCTgg > 1:1743415/1‑41 (MQ=255) ccGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCg < 1:1735290/71‑1 (MQ=255) gAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGAt > 1:571456/1‑69 (MQ=255) gAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATg < 1:1748865/70‑1 (MQ=255) gAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATg < 1:151055/70‑1 (MQ=255) gTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGt < 1:53179/71‑1 (MQ=255) gTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGt < 1:1862896/71‑1 (MQ=255) tCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCg < 1:137647/63‑1 (MQ=255) tCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCg < 1:286657/63‑1 (MQ=255) tCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCg < 1:1632000/63‑1 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:1936224/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:340392/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:1335913/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:471183/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:1331018/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:1533654/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:1240855/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTg > 1:162718/1‑52 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGa > 1:1837782/1‑63 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGa > 1:1093026/1‑63 (MQ=255) gATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGa > 1:1062249/1‑63 (MQ=255) gTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCt > 1:1195447/1‑71 (MQ=255) cAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTAtt < 1:1399176/40‑1 (MQ=255) aaaCGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAc < 1:969593/71‑1 (MQ=255) aaaCGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAc < 1:808564/71‑1 (MQ=255) aaaCGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAc < 1:526785/71‑1 (MQ=255) aaaCGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAc < 1:1837086/71‑1 (MQ=255) | TCAGCGATCGGCTGAACATCGATGTCGTACCCGTGGAGTCGATGGTCGATCAAATGGGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCAAAAC > W3110S.gb/3798106‑3798227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |