Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,4100TA100.0% 170.5 / NA 52pseudogene (543/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/38);  total (14/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGA  >  W3110S.gb/3824342‑3824463
                                                                    |                                                     
gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                                                     >  1:1068694/1‑71 (MQ=255)
gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                                                     >  1:204644/1‑71 (MQ=255)
gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                                                     >  1:754364/1‑71 (MQ=255)
gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                                                     >  1:1050529/1‑71 (MQ=255)
gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                                                     >  1:1761762/1‑71 (MQ=255)
            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         >  1:964083/1‑71 (MQ=255)
            tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         >  1:1814428/1‑71 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:306397/70‑1 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:333101/70‑1 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:808008/70‑1 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:1367448/70‑1 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:604600/70‑1 (MQ=255)
             cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:661770/70‑1 (MQ=255)
              tGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:623003/69‑1 (MQ=255)
                tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg                                     <  1:951522/71‑1 (MQ=255)
                tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATg                                     <  1:520148/71‑1 (MQ=255)
                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt                                <  1:1756102/70‑1 (MQ=255)
                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt                                <  1:1484930/70‑1 (MQ=255)
                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt                                <  1:1823251/70‑1 (MQ=255)
                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa                               <  1:741584/71‑1 (MQ=255)
                      ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa                               <  1:1244322/71‑1 (MQ=255)
                       tgtaAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTa                               <  1:1884211/70‑1 (MQ=255)
                        gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             >  1:1842642/4‑71 (MQ=255)
                        gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             >  1:984749/4‑71 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:736448/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:346574/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:950180/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:959386/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:28176/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:1201810/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:1212595/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:188808/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:1782225/68‑1 (MQ=255)
                         taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt                             <  1:1774011/68‑1 (MQ=255)
                                tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCg                     <  1:1657205/70‑1 (MQ=255)
                                tgAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCg                     <  1:1575758/70‑1 (MQ=255)
                                       atttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa                                         <  1:1430069/43‑1 (MQ=39)
                                          tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGCCCGACATGAACGTAGtg                            >  1:1898033/1‑54 (MQ=255)
                                          tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga                           >  1:277113/1‑55 (MQ=255)
                                          tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga                           >  1:1067330/1‑55 (MQ=255)
                                          tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtga                           >  1:974242/1‑55 (MQ=255)
                                          tGCGATCTGGAATACGTTGAAGACGCAGCCGCAGAGACCGACATGAACGTAGtga                           >  1:689436/1‑55 (MQ=255)
                                                   gAATACGTTGAAGACTGAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg   <  1:381702/70‑1 (MQ=255)
                                                   gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg   <  1:463138/70‑1 (MQ=255)
                                                   gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg   <  1:1327939/70‑1 (MQ=255)
                                                   gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg   <  1:347078/70‑1 (MQ=255)
                                                   gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg   <  1:1129125/70‑1 (MQ=255)
                                                          ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa  <  1:985998/64‑1 (MQ=255)
                                                          ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa  <  1:805865/64‑1 (MQ=255)
                                                          ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa  <  1:237503/64‑1 (MQ=255)
                                                          ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa  <  1:1923233/64‑1 (MQ=255)
                                                          ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa  <  1:1334501/64‑1 (MQ=255)
                                                                    |                                                     
GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGA  >  W3110S.gb/3824342‑3824463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: