Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,5240TG100.0% 62.2 / NA 18pseudogene (657/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/9);  total (9/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGC  >  W3110S.gb/3824483‑3824576
                                         |                                                      
ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTg                           >  1:1122289/1‑70 (MQ=255)
ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTg                           >  1:1468248/1‑70 (MQ=255)
ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTg                           >  1:508068/1‑70 (MQ=255)
           cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg                >  1:1223769/1‑70 (MQ=255)
           cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg                >  1:898460/1‑70 (MQ=255)
           cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg                >  1:1378064/1‑70 (MQ=255)
                   aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc        <  1:1635870/70‑1 (MQ=255)
                   aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc        <  1:1859102/70‑1 (MQ=255)
                   aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc        <  1:36103/70‑1 (MQ=255)
                   aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc        >  1:882222/1‑70 (MQ=255)
                        cTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTgg   >  1:1843286/1‑70 (MQ=255)
                        cTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc  <  1:1973190/71‑1 (MQ=255)
                        cTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc  <  1:1554768/71‑1 (MQ=255)
                        cTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc  <  1:709106/71‑1 (MQ=255)
                        cTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc  <  1:888908/71‑1 (MQ=255)
                         tACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc  <  1:1816501/70‑1 (MQ=255)
                                    ttgGCGCTGGCCCGA‑GGGGTAATCGAATCCATTGTAGCGACGCAGAAggcggc        <  1:1857188/53‑1 (MQ=255)
                                     tgGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGgcgc      >  1:80371/1‑54 (MQ=255)
                                         |                                                      
GGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGC  >  W3110S.gb/3824483‑3824576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑