| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,832,029 | A→G | 46.7% | intergenic (‑152/‑290) | kdtA ← / → rfaQ | 3‑deoxy‑D‑manno‑octulosonic‑acid transferase/lipopolysaccharide core biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,832,029 | 0 | A | G | 46.7% | ‑0.4 / 41.0 | 30 | intergenic (‑152/‑290) | kdtA/rfaQ | 3‑deoxy‑D‑manno‑octulosonic‑acid transferase/lipopolysaccharide core biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (4/12); new base G (4/10); total (8/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCATTTTCAAATCTACATAGGCCGTACTGACATTATCGA > W3110S.gb/3831960‑3832077 | aaaTAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGa < 1:1634532/70‑1 (MQ=255)aaaTAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGa < 1:260398/70‑1 (MQ=255)aaaTAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGa < 1:1883475/70‑1 (MQ=255)aaaTAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGa < 1:1249146/70‑1 (MQ=255) aGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGccc < 1:1338898/70‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:134335/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:258931/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:629123/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:1548379/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:1595089/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:1883770/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:409589/71‑1 (MQ=255) gTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:1821311/71‑1 (MQ=255) tGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCAttt > 1:1740989/1‑71 (MQ=255) tGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCAttt > 1:375176/1‑71 (MQ=255) tGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCAtt > 1:1362543/1‑70 (MQ=255) cTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCa < 1:101934/64‑1 (MQ=255) cTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCATTTTCAAATCTa > 1:330786/1‑71 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTa < 1:1225557/68‑1 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTa < 1:692480/68‑1 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa < 1:520588/70‑1 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa < 1:1848162/70‑1 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa < 1:838250/70‑1 (MQ=255) ccGCTACATCTTCATTTTTTTTGTATTCTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa < 1:383141/70‑1 (MQ=255) ccGCTACATCCTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTa < 1:1784831/68‑1 (MQ=255) gCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa > 1:178829/1‑68 (MQ=255) gCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa > 1:1759245/1‑68 (MQ=255) gCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa > 1:1394500/1‑68 (MQ=255) gCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACa > 1:645036/1‑68 (MQ=255) cATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACATAGGc < 1:129623/69‑1 (MQ=255) cATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACATAGGc < 1:891651/69‑1 (MQ=255) cATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGGAACTCGGCCCCATTTTCAAATCTACATAGGc < 1:96629/69‑1 (MQ=255) gTATTTTTATGCGATTCATTGAAACTCGGCCCCATTTTCAAATCTACATAGGCCGTACTGACATTATCGa < 1:1968555/70‑1 (MQ=255) gTATTTTTATGCGATTCATTGAAACTCGGCCCCATTTTCAAATCTACATAGGCCGTACTGACATTATCGa < 1:1364575/70‑1 (MQ=255) | AAATAGCTTCAGTTTGGTCTGATCTGCCGCTACATCTTCATTTTTTTTGTATTTTTATGCGATTCATTGAAACTCGGCCCCATTTTCAAATCTACATAGGCCGTACTGACATTATCGA > W3110S.gb/3831960‑3832077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |