Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,861,751	T→C	51.4%	N423S (AAC→AGC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,861,751	0	T	C	51.4%	5.3 / 110.2	74	N423S (AAC→AGC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (23/13);  new base C (11/27);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 4.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGT  >  W3110S.gb/3861690‑3861818                                                              |                                                                    ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCTGAATAGTTCGAAATa                                                             >  1:1003559/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                                              >  1:1894371/1‑69 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:1031569/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:479976/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:335681/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:1734721/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:76958/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:1625020/1‑70 (MQ=255) ggTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                             >  1:1221646/1‑70 (MQ=255)    gAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                                         >  1:169826/1‑71 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:1072193/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:882952/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:1766870/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:810863/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:1371639/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:1374247/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:344037/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:788742/1‑69 (MQ=255)             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGc                                                  >  1:1966859/1‑69 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:731805/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:1084528/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:1947031/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:1712817/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:305291/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:882058/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:115349/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:1575476/57‑1 (MQ=255)                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCg                                                       <  1:138868/57‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:351086/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:472505/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:1840519/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:1224366/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:880555/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:1528227/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:1644490/71‑1 (MQ=255)                       gcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:1882575/71‑1 (MQ=255)                        ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATAcc                                      <  1:237063/70‑1 (MQ=255)                           gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                                  <  1:1251901/71‑1 (MQ=255)                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                               >  1:671169/1‑71 (MQ=255)                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                               >  1:412260/1‑71 (MQ=255)                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                               >  1:413255/1‑71 (MQ=255)                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                               >  1:415804/1‑71 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:989526/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:881013/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:739292/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:69758/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:82434/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:616998/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:44338/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:1068154/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:440537/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:1050776/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATTCCAGCACCATACCGATGGAGTa                             <  1:721344/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:1027440/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:1588164/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:684405/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:656845/71‑1 (MQ=255)                                aGCGCCAGTGTTGATGACAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:494347/71‑1 (MQ=255)                                 gcgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                             <  1:578307/70‑1 (MQ=255)                                         gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                                        >  1:472024/1‑35 (MQ=255)                                         gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                                        >  1:1138901/1‑35 (MQ=255)                                         gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                                        >  1:1664545/1‑35 (MQ=255)                                         gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                                        >  1:129294/1‑35 (MQ=255)                                          ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATgg                        >  1:1889159/1‑66 (MQ=255)                                          ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                     >  1:894088/1‑69 (MQ=255)                                          ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                     >  1:737903/1‑69 (MQ=255)                                          ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                     >  1:842707/1‑69 (MQ=255)                                          ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                     >  1:1906624/1‑69 (MQ=255)                                                aCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                           >  1:172269/1‑57 (MQ=255)                                                aCAGTCCGGAATAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                           >  1:195030/1‑57 (MQ=255)                                                          aTAGTTCGAAATAAAGGCGAATGCCAGCACCTTACCGATGGAGTa                             <  1:473931/45‑1 (MQ=255)                                                           tAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGt  <  1:1309812/71‑1 (MQ=255)                                                           tAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGt  <  1:1313824/71‑1 (MQ=255)                                                           tAGCTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGt  <  1:693400/71‑1 (MQ=255)                                                              |                                                                    GGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGT  >  W3110S.gb/3861690‑3861818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑