| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,066,786 | C→T | 100% | intergenic (+144/‑129) | asd → / → glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,066,786 | 0 | C | T | 80.8% | 52.7 / 9.0 | 26 | intergenic (+144/‑129) | asd/glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base C (5/0); new base T (8/13); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAAAA > W3110S.gb/4066731‑4066851 | ttAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAg < 1:334068/71‑1 (MQ=255)ttAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCCTACCTGAAGTGAg < 1:197364/71‑1 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg < 1:444359/70‑1 (MQ=255) ttCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATg < 1:1883584/70‑1 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAgg > 1:124287/1‑65 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:1446890/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:995055/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:1260340/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:207940/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:1604313/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:1548559/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTCAGGACGACGCAGAGAGGATGca > 1:1625237/1‑70 (MQ=255) cTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:880401/1‑70 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:1589285/70‑1 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:571123/70‑1 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACag < 1:960015/70‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgct > 1:228296/1‑71 (MQ=255) ggCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgctgc > 1:1457975/1‑71 (MQ=255) ggCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgctgc > 1:1072169/1‑71 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:1330519/39‑1 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:1273372/39‑1 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:709757/39‑1 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:733206/39‑1 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:823814/39‑1 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:140575/39‑1 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaaaa > 1:782860/1‑71 (MQ=255) | TTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAAAA > W3110S.gb/4066731‑4066851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |