Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 344,381 | G→A | 32.0% | intergenic (+166/‑247) | yahL → / → yahM | hypothetical protein/hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 344,381 | 0 | G | A | 32.0% | 65.5 / 42.1 | 50 | intergenic (+166/‑247) | yahL/yahM | hypothetical protein/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base G (15/19); new base A (12/4); total (27/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.73e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA > W3110S.gb/344314‑344445 | ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:317264/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1745596/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1664966/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1623443/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1778448/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:180121/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:412569/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:682428/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:116913/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:740524/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGttt > 1:1108178/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1914765/1‑71 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1041384/1‑71 (MQ=255) ttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1654053/71‑1 (MQ=255) ttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1754224/71‑1 (MQ=255) aTCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:136254/70‑1 (MQ=255) aTCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1382098/70‑1 (MQ=255) aTCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:652242/70‑1 (MQ=255) aTCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1842109/70‑1 (MQ=255) aTCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1550241/70‑1 (MQ=255) cGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATg < 1:54322/64‑1 (MQ=255) gACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAg < 1:1794840/71‑1 (MQ=255) gACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAg < 1:154015/71‑1 (MQ=255) gACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAg < 1:1435510/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:1854746/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:1902589/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:1501711/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:1939281/71‑1 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:97103/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:892864/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1208123/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:332542/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:570827/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:168979/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:743015/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1162897/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:293634/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:508368/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:42825/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1538573/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1535920/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATCCAGATGtt > 1:757878/1‑70 (MQ=255) aaaaaaGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:1272674/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:120130/69‑1 (MQ=255) aaaaaaGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:350767/69‑1 (MQ=255) aaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAAtgt < 1:787063/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:784693/71‑1 (MQ=255) gtagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:1728463/71‑1 (MQ=255) tagtGGTTTCTCAGCCATGCGTTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGa < 1:1002474/70‑1 (MQ=255) tagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1493408/39‑1 (MQ=255) | CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA > W3110S.gb/344314‑344445 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |