| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,301,561 | G→A | 24.5% | D429D (GAC→GAT) | fdhF ← | formate dehydrogenase‑H, selenopolypeptide subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,301,561 | 0 | G | A | 24.5% | 93.1 / 28.7 | 49 | D429D (GAC→GAT) | fdhF | formate dehydrogenase‑H, selenopolypeptide subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/25); new base A (2/10); total (14/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
CATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTG > W3110S.gb/4301494‑4301629 | cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1970494/1‑71 (MQ=255)cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:227414/1‑71 (MQ=255)cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1327687/1‑71 (MQ=255)cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1337641/1‑71 (MQ=255)cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:571442/1‑71 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:412802/71‑1 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1688316/71‑1 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1185168/71‑1 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1246938/71‑1 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:825698/71‑1 (MQ=255) tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1368213/71‑1 (MQ=255) gCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAAc < 1:1843014/69‑1 (MQ=255) ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1366027/65‑1 (MQ=255) ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1812299/65‑1 (MQ=255) ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1961421/65‑1 (MQ=255) ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:58832/65‑1 (MQ=255) gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1919853/1‑68 (MQ=255) gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1387296/1‑68 (MQ=255) gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1175911/1‑68 (MQ=255) gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:116864/1‑68 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAg < 1:653263/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAg < 1:849737/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:390978/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1899036/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:484194/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:168768/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:532422/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1532908/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1530413/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1489510/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1137450/70‑1 (MQ=255) aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTACCAg < 1:1124943/70‑1 (MQ=255) aaTAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAc < 1:1601519/54‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:851219/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:396958/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1017355/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:262435/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1857017/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1295052/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:734631/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1624141/70‑1 (MQ=255) cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:586034/70‑1 (MQ=255) gccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACg < 1:76551/71‑1 (MQ=255) gccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACg < 1:547938/71‑1 (MQ=255) gcgGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGa > 1:980315/1‑71 (MQ=255) gcgGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGa > 1:524719/1‑71 (MQ=255) aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCa > 1:566071/1‑70 (MQ=255) aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCa > 1:412557/1‑70 (MQ=255) aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAg > 1:357872/1‑71 (MQ=255) gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:648759/1‑69 (MQ=255) gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:1394097/1‑69 (MQ=255) gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:1651206/1‑69 (MQ=255) gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:32208/1‑69 (MQ=255) gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:971224/1‑69 (MQ=255) | CATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTG > W3110S.gb/4301494‑4301629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |