| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,308,687 | G→A | 34.3% | I464I (ATC→ATT) | yjcS ← | predicted alkyl sulfatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,308,687 | 0 | G | A | 34.3% | 37.6 / 30.8 | 35 | I464I (ATC→ATT) | yjcS | predicted alkyl sulfatase |
| Reads supporting (aligned to +/- strand): ref base G (21/2); new base A (2/10); total (23/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
ACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAT > W3110S.gb/4308622‑4308754 | aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtgatga < 1:693/70‑1 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1802853/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1677081/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:955820/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1072977/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:908937/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1139564/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:1899474/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:695927/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:508714/1‑70 (MQ=255)aCCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTtaatga > 1:377466/1‑70 (MQ=255) cTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGcc > 1:403140/1‑70 (MQ=255) gTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGt < 1:433610/71‑1 (MQ=255) cACCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGACGAGCGCCTGCACGTAACGt > 1:1101062/1‑71 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:102523/1‑71 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:1165509/1‑71 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt > 1:100918/1‑71 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:390320/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1951422/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:972826/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1744583/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1681212/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1488266/71‑1 (MQ=255) aCCTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1549258/71‑1 (MQ=255) ccTTGCTTGTTCGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGtt < 1:1349535/70‑1 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGACCCGCCCAGCGCCTGCACGTAACGTTTa > 1:1652230/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGttt > 1:233069/1‑70 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1066352/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:477044/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1313336/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1461002/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:249628/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1572794/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1297295/1‑71 (MQ=255) cttgcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTa > 1:1785980/1‑71 (MQ=255) cGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTcc < 1:307419/70‑1 (MQ=255) cGCTTCTTGCGCCAGGTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTcc < 1:526482/70‑1 (MQ=255) cgcCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCa < 1:1755373/70‑1 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCCTCTCCACCTGACCATACGGAt > 1:1390311/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:607115/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1850528/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:759781/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:10800/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1686939/1‑71 (MQ=255) gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:1038937/1‑71 (MQ=255) gTTAATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAt > 1:541293/1‑71 (MQ=255) | ACCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGAT > W3110S.gb/4308622‑4308754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |