| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,414,112 | G→A | 18.9% | L52L (TTG→TTA) | rlmB → | 23S rRNA (Gm2251)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,414,112 | 0 | G | A | 18.9% | 83.8 / 13.0 | 37 | L52L (TTG→TTA) | rlmB | 23S rRNA (Gm2251)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (20/10); new base A (3/4); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
GGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCG > W3110S.gb/4414044‑4414170 | ggCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTgg > 1:1504421/1‑70 (MQ=255) aGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGcc < 1:1027938/71‑1 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:393884/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:6592/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:896048/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:1538127/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:935358/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:1198543/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:975995/1‑71 (MQ=255) aTAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCaa > 1:1069893/1‑71 (MQ=255) tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgac > 1:1263303/1‑71 (MQ=255) tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgac > 1:967683/1‑71 (MQ=255) tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgac > 1:1591543/1‑71 (MQ=255) tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgac > 1:705715/1‑71 (MQ=255) tGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTcgac > 1:137069/1‑71 (MQ=255) gAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTg < 1:1753479/69‑1 (MQ=255) tctcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCAt > 1:932690/1‑69 (MQ=255) tctcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCAt > 1:702642/1‑69 (MQ=255) tctcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCAt > 1:243470/1‑69 (MQ=255) tctcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGt < 1:1715096/60‑1 (MQ=255) tctcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGt < 1:513298/60‑1 (MQ=255) tctcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGt < 1:534035/60‑1 (MQ=255) tctcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGt < 1:584144/60‑1 (MQ=255) tcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAgg > 1:1028876/1‑71 (MQ=255) tcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAgg > 1:381358/1‑71 (MQ=255) tcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAgg > 1:1780481/1‑71 (MQ=255) tcAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAgg > 1:1565067/1‑71 (MQ=255) tcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATGTCTCGACGAGAAAAGCGACGGTGc > 1:1627680/1‑60 (MQ=255) tcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGc > 1:887565/1‑60 (MQ=255) tcAGGGCGTGGTTATCCAGTTAGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGc > 1:1241152/1‑60 (MQ=255) aGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAggg < 1:486136/70‑1 (MQ=255) aGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAggg < 1:25794/70‑1 (MQ=255) ggCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAggg < 1:17402/68‑1 (MQ=255) gTTATCCTGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg < 1:1917331/70‑1 (MQ=255) gTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg < 1:469667/70‑1 (MQ=255) gTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg < 1:1440839/70‑1 (MQ=255) gTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCg < 1:117511/70‑1 (MQ=255) cAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAggg < 1:15112/56‑1 (MQ=255) | GGCCGTGAAGATAAACGTCTGTTACCGCTGATTCACGCCCTTGAGTCTCAGGGCGTGGTTATCCAGTTGGCAAACCGCCAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCG > W3110S.gb/4414044‑4414170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |