| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,491,067 | G→A | 100% | V56V (GTG→GTA) | yjgP → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,491,067 | 0 | G | A | 96.2% | 78.1 / ‑3.6 | 26 | V56V (GTG→GTA) | yjgP | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (6/19); total (6/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAC > W3110S.gb/4491013‑4491123 | ggTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGc < 1:1875134/71‑1 (MQ=255) gTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCt < 1:357735/71‑1 (MQ=255) ttGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCt < 1:1602155/70‑1 (MQ=255) ttCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:669061/46‑1 (MQ=255) ttCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:1505797/46‑1 (MQ=255) ttCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:1686319/46‑1 (MQ=255) tCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:1838185/45‑1 (MQ=255) ggCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:136926/42‑1 (MQ=255) tctcCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGc < 1:1027448/39‑1 (MQ=255) ccTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgctgct < 1:1198209/69‑1 (MQ=255) ccTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgctgct < 1:1758278/69‑1 (MQ=255) ccTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgctgct < 1:1849142/69‑1 (MQ=255) ccTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgctgct < 1:1443479/69‑1 (MQ=255) cTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCtgat < 1:970241/71‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:1964865/36‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:347714/36‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:1328866/36‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:390396/36‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:533369/36‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa < 1:646913/36‑1 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATc > 1:966870/1‑36 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCtgatga > 1:451101/1‑70 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAc > 1:1614856/1‑71 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAc > 1:1199922/1‑71 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAc > 1:733547/1‑71 (MQ=255) ctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAc > 1:758758/1‑71 (MQ=255) | GGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGAC > W3110S.gb/4491013‑4491123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |