Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,528,968 A→G 100% L242P (CTA→CCA)  yjhH ← predicted lyase/synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,528,9680AG95.0% 67.0 / ‑4.0 20L242P (CTA→CCA) yjhHpredicted lyase/synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (3/16);  minor base T (1/0);  total (4/16)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGA  >  W3110S.gb/4528908‑4529025
                                                            |                                                         
cTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTTGTTTTTTAtt                                                 >  1:137398/1‑71 (MQ=255)
cTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTAtt                                                 >  1:1416362/1‑71 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCa                             >  1:915244/1‑61 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCa                             >  1:317140/1‑61 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:169930/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:611161/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1939813/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1772080/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1317006/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1038235/70‑1 (MQ=255)
                              tCGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1493861/70‑1 (MQ=255)
                               cGAGGGCGTAAATAGCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCtt                    <  1:1414963/69‑1 (MQ=255)
                                             gctggCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:1750310/68‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:124399/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:1575136/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:622107/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:388537/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:1606544/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:1760674/71‑1 (MQ=255)
                                             gCGGGCAGTTGTAGTGGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTa    <  1:1686881/71‑1 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:1618586/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:1008080/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:1227647/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:1692039/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:633827/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:792149/1‑63 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGACTTCACGCCAGgcgc        >  1:353347/1‑57 (MQ=255)
                                                       gtagtgGTTTTTTATTCAGCCTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGa  >  1:524182/1‑63 (MQ=255)
                                                            |                                                         
CTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGA  >  W3110S.gb/4528908‑4529025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑