| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 419,576 | G→A | 61.5% | A254A (GCG→GCA) | brnQ → | predicted branched chain amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 419,576 | 0 | G | A | 61.5% | 15.3 / 26.8 | 26 | A254A (GCG→GCA) | brnQ | predicted branched chain amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (4/6); new base A (7/9); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGG > W3110S.gb/419518‑419641 | cGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGAt < 1:1374361/71‑1 (MQ=255)cGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGAt < 1:302429/71‑1 (MQ=255)cGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGAt < 1:453909/71‑1 (MQ=255) gtgtTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAg > 1:895504/1‑71 (MQ=255) gtgtTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAg > 1:620139/1‑71 (MQ=255) tACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTa < 1:1323139/70‑1 (MQ=255) tACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTa < 1:1470276/70‑1 (MQ=255) tACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTa < 1:1689277/70‑1 (MQ=255) tACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTa < 1:598595/70‑1 (MQ=255) tACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTa < 1:49029/70‑1 (MQ=255) ggCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCt > 1:626891/1‑69 (MQ=255) ggCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg > 1:950413/1‑70 (MQ=255) ggCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg > 1:761818/1‑70 (MQ=255) ggCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg > 1:513715/1‑70 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1400945/69‑1 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:362964/69‑1 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:206508/69‑1 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1754126/69‑1 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1659348/69‑1 (MQ=255) gcgcTGTTCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1588430/69‑1 (MQ=255) ttCCGTCTGGGGTCAGACAGCGCATCGCTGGTCGAt < 1:1407964/36‑1 (MQ=255) aGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTgg > 1:299174/1‑71 (MQ=255) aGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTgg > 1:1380035/1‑71 (MQ=255) aGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTgg > 1:332631/1‑71 (MQ=255) aGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTgg > 1:30351/1‑71 (MQ=255) aGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTgg > 1:488735/1‑71 (MQ=255) | CGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGG > W3110S.gb/419518‑419641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |