| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,548,379 | G→A | 73.1% | intergenic (+34/‑31) | fimA → / → fimI | major type 1 subunit fimbrin/fimbrial protein involved in type 1 pilus biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,548,379 | 0 | G | A | 73.1% | 39.0 / 15.6 | 26 | intergenic (+34/‑31) | fimA/fimI | major type 1 subunit fimbrin/fimbrial protein involved in type 1 pilus biosynthesis |
| Reads supporting (aligned to +/- strand): ref base G (5/2); new base A (10/9); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
ATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTT > W3110S.gb/4548311‑4548444 | aTGTGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:402740/69‑1 (MQ=255)aTGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:770568/69‑1 (MQ=255)aTGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:1786259/69‑1 (MQ=255)aTGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:1847350/69‑1 (MQ=255)aTGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:463973/69‑1 (MQ=255) cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGc > 1:1030913/1‑71 (MQ=255) cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGc > 1:668436/1‑71 (MQ=255) cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGc > 1:57879/1‑71 (MQ=255) cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGc > 1:1896620/1‑71 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGa > 1:768393/1‑70 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGa > 1:497390/1‑70 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGAt > 1:1035057/1‑71 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGAt > 1:832217/1‑71 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGAt > 1:703773/1‑71 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGAt > 1:1956581/1‑71 (MQ=255) aaTAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGAt > 1:1645389/1‑71 (MQ=255) tAACCTACCCAGGTTCAGGGACGTCATTACGGGCAgg < 1:1199964/37‑1 (MQ=255) gTTCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGat < 1:301781/71‑1 (MQ=255) tCAGGGACGTCATTACGGGCAGAGATGCCCTCCCTTGTGCGATAAAAAAAACGATGAAAAGGAag > 1:78018/1‑65 (MQ=255) tCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGatta > 1:1724678/1‑71 (MQ=255) tCAGGGACGTCATTACGGGCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGatt > 1:1965996/1‑70 (MQ=255) cATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAg > 1:604441/1‑71 (MQ=255) cGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgtc < 1:939949/70‑1 (MQ=255) cGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgtc < 1:1442538/70‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:1809478/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:1891778/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:735576/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:557004/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:506468/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:503972/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:221615/71‑1 (MQ=255) ggCAGAGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt < 1:37842/71‑1 (MQ=255) | ATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTT > W3110S.gb/4548311‑4548444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |