| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 505,179 | C→T | 68.3% | L348L (CTG→TTG) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 505,179 | 0 | C | T | 68.3% | 48.5 / 34.2 | 41 | L348L (CTG→TTG) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base C (2/11); new base T (12/16); total (14/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAA > W3110S.gb/505125‑505225 | agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:154474/1‑69 (MQ=255)agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:1162300/1‑69 (MQ=255)agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:1795462/1‑69 (MQ=255)agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:1390726/1‑69 (MQ=255)agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:1243705/1‑69 (MQ=255)agcgagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:190013/1‑69 (MQ=255) gcgcGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACa < 1:991161/71‑1 (MQ=255) gcgcGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACa < 1:627576/71‑1 (MQ=255) cgcgTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACa < 1:1391169/70‑1 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1302934/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1349099/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1871699/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1815333/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:651091/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1663909/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1732515/1‑71 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1672894/1‑71 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCATAGCGCAGCTGGAAGTGaa < 1:550376/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:220491/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:932623/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:861552/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:123362/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:563650/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:407435/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:366099/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1554187/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1396281/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1445903/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1468656/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1028561/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1491074/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1848987/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:528330/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1483405/71‑1 (MQ=255) gCTGAAAACCAGCAAACGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1652348/71‑1 (MQ=255) cTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1329325/70‑1 (MQ=255) cTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1173189/70‑1 (MQ=255) aTCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1127570/53‑1 (MQ=255) aTCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:937215/53‑1 (MQ=255) aTCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:347179/53‑1 (MQ=255) aTCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1954223/53‑1 (MQ=255) | AGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAA > W3110S.gb/505125‑505225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |