| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 515,934 | C→T | 100% | A43V (GCG→GTG) | ybbM → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 515,934 | 0 | C | T | 81.3% | 32.1 / 1.8 | 16 | A43V (GCG→GTG) | ybbM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (2/11); total (2/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAG > W3110S.gb/515867‑515997 | gCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGgcgagtga > 1:1391531/1‑70 (MQ=255)gCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGgcgagtga > 1:327969/1‑70 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGt > 1:1802993/1‑70 (MQ=255) aaaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc < 1:1945811/71‑1 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc < 1:402150/70‑1 (MQ=255) aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGc < 1:1738259/70‑1 (MQ=255) aaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTa < 1:1431336/71‑1 (MQ=255) aaCTTGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:1901305/58‑1 (MQ=255) aaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:949899/58‑1 (MQ=255) aaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:353370/58‑1 (MQ=255) aaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:1566845/58‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:274169/57‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTa < 1:646328/69‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAtg < 1:517148/71‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAtg < 1:1305913/71‑1 (MQ=255) cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatta > 1:272380/1‑58 (MQ=255) gggCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAg < 1:1874652/71‑1 (MQ=255) gggCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAg < 1:796533/71‑1 (MQ=255) | GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAG > W3110S.gb/515867‑515997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |