| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 531,446 | C→T | 28.0% | intergenic (‑1/‑229) | ybbS ← / → allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 531,446 | 0 | C | T | 28.0% | 36.9 / 15.4 | 25 | intergenic (‑1/‑229) | ybbS/allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (12/6); new base T (3/4); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
CTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTT > W3110S.gb/531382‑531501 | cTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAgg < 1:1900243/71‑1 (MQ=255) gAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATc < 1:1148877/70‑1 (MQ=255) gCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt < 1:1716360/71‑1 (MQ=255) gCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt > 1:1680903/1‑71 (MQ=255) gCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt > 1:1664926/1‑71 (MQ=255) gCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt < 1:1378802/71‑1 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1847505/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:963330/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:869015/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:674102/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:357941/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1832696/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1787729/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1076488/1‑71 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1414531/1‑71 (MQ=255) aaaGTCCGCAAGGTTCCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTc > 1:1299067/1‑71 (MQ=255) gTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:1707594/71‑1 (MQ=255) gTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:1347154/71‑1 (MQ=255) gTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:628181/71‑1 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:67045/70‑1 (MQ=255) gTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGAt > 1:1620658/1‑71 (MQ=255) gTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGAt > 1:1533611/1‑71 (MQ=255) gTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCATCTGAt > 1:603490/1‑71 (MQ=255) tCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGAttt < 1:1973136/70‑1 (MQ=255) aaCATCTTCAGGTATCCCCTTTTAAATCCGCAAGt < 1:1280857/35‑1 (MQ=255) | CTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTT > W3110S.gb/531382‑531501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |