| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 746,293 | C→T | 39.3% | A236V (GCC→GTC) | ybgL → | predicted lactam utilization protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 746,293 | 0 | C | T | 39.3% | 25.4 / 41.3 | 39 | A236V (GCC→GTC) | ybgL | predicted lactam utilization protein |
| Reads supporting (aligned to +/- strand): ref base C (6/17); new base T (12/3); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTG > W3110S.gb/746235‑746363 | ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:968437/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:960119/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:4126/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:852570/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:1812894/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:5837/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:1554394/71‑1 (MQ=255)ctgcATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:774509/71‑1 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:446481/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:44140/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:490197/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:1899612/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:1854196/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:683122/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:772623/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:1406291/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:890587/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:942225/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:1221627/1‑71 (MQ=255) gCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTc > 1:1053630/1‑71 (MQ=255) cGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCAt < 1:841856/71‑1 (MQ=255) ggCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGTTTGTTGTCGCAGCATAACCCCGATtaa > 1:1482879/1‑71 (MQ=255) tCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGAt < 1:1759252/40‑1 (MQ=255) cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg < 1:985027/70‑1 (MQ=255) cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg < 1:1328247/70‑1 (MQ=255) cgcccgccGACTACGCTCTGCATTTGTCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAg < 1:449384/70‑1 (MQ=255) cgcccgccGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATtaa > 1:571403/1‑65 (MQ=255) cATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCAc < 1:1460690/69‑1 (MQ=255) cATTTGCCGAAAAAGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGata > 1:1092175/1‑65 (MQ=255) cATTTGCCGAAAAAGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGata > 1:1656249/1‑65 (MQ=255) cATTTGCCGAAAAAGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGata > 1:592052/1‑65 (MQ=255) cATTTGCCGAAAAAGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGata > 1:1760895/1‑65 (MQ=255) cATTTGACGAAAAAGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAg > 1:1749475/1‑61 (MQ=255) aTTTGCCGAAAAGGGGATTTTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:1125846/71‑1 (MQ=255) aTTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:6588/71‑1 (MQ=255) aTTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:1360833/71‑1 (MQ=255) aTTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:461946/71‑1 (MQ=255) aTTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATAACCAt < 1:530629/71‑1 (MQ=255) tttGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCAt < 1:200236/70‑1 (MQ=255) ccGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTg < 1:1796293/71‑1 (MQ=255) ccGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTg < 1:1389318/71‑1 (MQ=255) ccGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTg < 1:884443/71‑1 (MQ=255) ccGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTg < 1:1203561/71‑1 (MQ=255) | CTGCATGGCGACGGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATTGTTGTCGCAGCATAACCCCGATTAATAAAGAATGAAAAAAGGATATCACCATGCCTG > W3110S.gb/746235‑746363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |