Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 795,129 C→T 65.5% intergenic (‑63/‑66) modE ← / → ybhT DNA‑binding transcriptional dual regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb795,1290CT65.5% 27.0 / 26.2 29intergenic (‑63/‑66)modE/ybhTDNA‑binding transcriptional dual regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base T (10/9);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAC  >  W3110S.gb/795064‑795177
                                                                 |                                                
cATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAg                                             >  1:207840/1‑71 (MQ=255)
cATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAg                                             >  1:39158/1‑71 (MQ=255)
cATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAg                                             >  1:194265/1‑71 (MQ=255)
cATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAg                                             >  1:187602/1‑71 (MQ=255)
cATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAg                                             >  1:723494/1‑71 (MQ=255)
    aCAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAggg                                           >  1:1150085/1‑69 (MQ=255)
    aCAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAggg                                           >  1:1791278/1‑69 (MQ=255)
    aCAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAggg                                           >  1:1469994/1‑69 (MQ=255)
    aCAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAggg                                           >  1:932721/1‑69 (MQ=255)
    aCAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAgg                                            >  1:1774390/1‑68 (MQ=255)
            cTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCttt                                  <  1:1506913/70‑1 (MQ=255)
            cTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCttt                                  <  1:1494639/70‑1 (MQ=255)
            cTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCttt                                  <  1:1794594/70‑1 (MQ=255)
            cTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCttt                                  <  1:427715/70‑1 (MQ=255)
            cTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCttt                                  <  1:1322334/70‑1 (MQ=255)
              ggCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCt                               >  1:702375/1‑71 (MQ=255)
              ggCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCt                               >  1:672477/1‑71 (MQ=255)
              ggCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCt                               >  1:1281771/1‑71 (MQ=255)
              ggCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCt                               >  1:1960104/1‑71 (MQ=255)
              ggCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCt                               >  1:193870/1‑71 (MQ=255)
                                aCGGAAAACGAACGCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGaa              <  1:81228/70‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:201401/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:1831637/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:695347/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:1727136/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:865936/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:1373113/71‑1 (MQ=255)
                                           acgCCACGCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:961802/71‑1 (MQ=255)
                                                  gCAAAGCTGACCGCATAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAc  <  1:1585524/64‑1 (MQ=255)
                                                                 |                                                
CATAACAATGTCCTGGCAAAAGTCTTATTGTGACGGAAAACGAACGCCACGCAAAGCTGACCGCACAAAAGGGGAGTGCTTTTCTGTGCTTAGCGGTTAGAATAGTCTCATGAC  >  W3110S.gb/795064‑795177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑