| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 827,597 | C→T | 100% | P26P (CCG→CCA) | ybhS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 827,597 | 0 | C | T | 88.9% | 99.1 / 5.0 | 36 | P26P (CCG→CCA) | ybhS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base T (25/7); total (29/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCG > W3110S.gb/827534‑827664 | aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:1869554/1‑71 (MQ=255)aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:150446/1‑71 (MQ=255)aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:1508478/1‑71 (MQ=255)aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgc > 1:283443/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:1473453/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:242283/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:1586621/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:482095/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:465652/1‑71 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:1714083/1‑71 (MQ=255) aCCGCAATCAGCCAGCTACTCGGATCGCGAACGATCt > 1:1178131/1‑37 (MQ=255) aCCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:1960004/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:1237998/1‑70 (MQ=255) aCCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc > 1:1235733/1‑70 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:747908/69‑1 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:781332/69‑1 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:219542/69‑1 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:1784852/69‑1 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:1672211/69‑1 (MQ=255) ccGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:217285/69‑1 (MQ=255) cGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:483198/68‑1 (MQ=255) tACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATc > 1:951288/1‑71 (MQ=255) tACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATc > 1:287989/1‑71 (MQ=255) tACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATc > 1:229568/1‑71 (MQ=255) tACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATc > 1:1662919/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGAc > 1:73672/1‑56 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:481817/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1542036/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:778334/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:754073/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1552065/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:409498/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:294037/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1775355/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:246690/1‑71 (MQ=255) aCTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCg > 1:1775389/1‑71 (MQ=255) | AATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCG > W3110S.gb/827534‑827664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |