| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 906,962 | G→A | 52.2% | H72Y (CAC→TAC) | ybjS ← | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 906,962 | 0 | G | A | 52.2% | ‑0.3 / 37.5 | 29 | H72Y (CAC→TAC) | ybjS | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (2/11); new base A (2/13); total (5/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGATCCGCCGG > W3110S.gb/906904‑907031 | aCGTTAGCCATATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATc < 1:928114/71‑1 (MQ=255)aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATc < 1:1051729/71‑1 (MQ=255)aCGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATc < 1:180752/71‑1 (MQ=255) gttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCAc > 1:771371/1‑67 (MQ=255) gttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACt > 1:1443220/1‑68 (MQ=255) gttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTCCCACAGCGTATCAATGCCCGCGAGCATCACtt > 1:937622/1‑69 (MQ=255) gtgtCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCt > 1:946277/1‑71 (MQ=255) gtgtCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCt > 1:73948/1‑71 (MQ=255) tCCCCCAGTGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1728075/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1877181/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:29544/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1942312/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1746232/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1653644/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1644558/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1391010/71‑1 (MQ=255) tCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:1723440/71‑1 (MQ=255) tGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:433141/61‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCTCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:248190/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:1415813/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:1075064/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:527376/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:63480/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:1184247/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:1302918/71‑1 (MQ=255) gTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTc < 1:1255072/71‑1 (MQ=255) gAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGa < 1:107398/69‑1 (MQ=255) gAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGa < 1:488175/69‑1 (MQ=255) gAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGa < 1:333223/69‑1 (MQ=255) gCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGATCCGCCgg > 1:655609/1‑70 (MQ=255) | ACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAACCAGCTCGGTCAGATCCGCCGG > W3110S.gb/906904‑907031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |