| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 978,430 | A→G | 26.5% | E561E (GAA→GAG) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 978,430 | 0 | A | G | 26.5% | 53.6 / 21.7 | 34 | E561E (GAA→GAG) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base G (4/5); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATGG > W3110S.gb/978381‑978491 | gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctct > 1:1092460/1‑71 (MQ=255)gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctct > 1:1644578/1‑71 (MQ=255)gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctc > 1:329053/1‑70 (MQ=255) atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:519522/71‑1 (MQ=255) atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:858278/71‑1 (MQ=255) atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:242406/71‑1 (MQ=255) atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:259917/71‑1 (MQ=255) atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:314359/71‑1 (MQ=255) tatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:50942/70‑1 (MQ=255) acgaACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGGGTCt < 1:1307504/65‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:143709/71‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:649601/71‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:838034/71‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:517053/71‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:932633/71‑1 (MQ=255) gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:1014965/71‑1 (MQ=255) cTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCAt > 1:838896/1‑71 (MQ=255) cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:1307752/70‑1 (MQ=255) cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:739023/70‑1 (MQ=255) cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:987139/70‑1 (MQ=255) cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:1532135/70‑1 (MQ=255) aTCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgt < 1:426534/69‑2 (MQ=255) aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:196792/1‑63 (MQ=255) aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:1681708/1‑63 (MQ=255) aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:144365/1‑63 (MQ=255) aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:752886/1‑63 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1402878/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:541891/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:457310/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:227754/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1853221/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1546712/1‑41 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCg > 1:801269/1‑39 (MQ=255) aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGAgtg > 1:999756/1‑41 (MQ=255) | GTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATGG > W3110S.gb/978381‑978491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |