Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 47,828 A→G 62.2% V20V (GTA→GTG kefC → potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb47,8280AG62.2% 64.9 / 80.2 74V20V (GTA→GTGkefCpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (19/9);  new base G (22/24);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGC  >  W3110S.gb/47761‑47894
                                                                   |                                                                  
ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAccc                                                                 <  1:389375/71‑1 (MQ=255)
 gCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCTGTTCGGCAGCGCTGATTGTAccc                                                                 <  1:284889/70‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                            <  1:881147/70‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                            <  1:445733/70‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                  <  1:210843/64‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                  <  1:1473304/64‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                  <  1:770076/64‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                  <  1:801349/64‑1 (MQ=255)
      tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGCTCGGCAGCGCTGATTGTGCCCATTGc                                                            <  1:1606857/70‑1 (MQ=255)
      tcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                            <  1:1371725/70‑1 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:1029045/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:718772/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:1767860/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:780362/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:1823642/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:1374834/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:353113/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:1955397/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:352606/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:1969028/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:693652/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:1715518/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:16567/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:1622516/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCa                                                                >  1:1649959/1‑64 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTg                                                             >  1:1763332/1‑67 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                          >  1:311563/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                          >  1:1859244/1‑70 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCGCTGAATTATCTCGGTTCGGCAGCGCTGATTGTGCCCAtt                                                              >  1:368281/1‑66 (MQ=255)
        atGGATAGCCATACGCTGATTCAGGCACTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                          >  1:28745/1‑70 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                            >  1:46846/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                            >  1:172194/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                            >  1:815222/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                            >  1:1227060/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCt                                             >  1:229785/1‑70 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:1863587/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:1123859/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:874782/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:1210825/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:1385245/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                            >  1:710882/1‑71 (MQ=255)
                     cGCTGATTCAGGCGCTGATTTATATCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                          >  1:971152/1‑57 (MQ=255)
                            tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:1910479/1‑71 (MQ=255)
                            tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:442858/1‑71 (MQ=255)
                            tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:1783666/1‑71 (MQ=255)
                            tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:1538419/1‑71 (MQ=255)
                            tCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:447644/1‑71 (MQ=255)
                             cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                            >  1:728797/1‑47 (MQ=255)
                             cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:1377412/1‑70 (MQ=255)
                             cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                     >  1:1967877/1‑70 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:11023/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1888333/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1868595/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1206675/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1751354/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1390072/70‑1 (MQ=255)
                                           tctcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1452258/70‑1 (MQ=255)
                                                      cAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGc           <  1:1128985/71‑1 (MQ=255)
                                                       aGCGATGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                       <  1:1210344/58‑1 (MQ=255)
                                                        tcgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1707511/70‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1671277/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:988352/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1437021/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1469053/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1559814/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:264153/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1675323/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:654473/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1717767/71‑1 (MQ=255)
                                                        gcgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1946069/71‑1 (MQ=255)
                                                        gcgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1829143/71‑1 (MQ=255)
                                                        gcgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:1203604/71‑1 (MQ=255)
                                                         cgcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat         <  1:90379/70‑1 (MQ=255)
                                                               ttGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGAACTTGGCTACCTGATCGCCGGCTGCATTATTGGc  >  1:1924922/1‑71 (MQ=255)
                                                                   |                                                                  
GGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGC  >  W3110S.gb/47761‑47894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: