Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 997,073 C→T 100% L98L (TTG→TTA)  ssuA ← alkanesulfonate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb997,0730CT100.0% 24.9 / NA 9L98L (TTG→TTA) ssuAalkanesulfonate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/0);  total (9/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGG  >  W3110S.gb/997016‑997095
                                                         |                      
ttCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCgg           >  1:1704631/1‑71 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGcc               >  1:1379567/1‑57 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1029362/1‑70 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1181143/1‑70 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1606474/1‑70 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1917231/1‑70 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1945425/1‑70 (MQ=255)
          aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:413746/1‑70 (MQ=255)
          aGAATAACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACTAAATCAGCCCCGGCAGCCTggg  >  1:1388873/1‑70 (MQ=255)
                                                         |                      
TTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGG  >  W3110S.gb/997016‑997095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑