Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,020,867 A→G 100% S159P (TCC→CCC)  sulA ← SOS cell division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,020,8670AG93.3% 43.6 / ‑3.6 15S159P (TCC→CCC) sulASOS cell division inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (8/6);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT  >  W3110S.gb/1020808‑1020927
                                                           |                                                            
ttCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCg                                                   <  1:588923/71‑1 (MQ=255)
         tAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGt                                             >  1:1007847/1‑68 (MQ=255)
                 aaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgc                                   <  1:423225/70‑1 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:1046210/1‑70 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:1385214/1‑70 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:247290/1‑70 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:32266/1‑70 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:338646/1‑70 (MQ=255)
                    ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt                                >  1:496080/1‑70 (MQ=255)
                              aTACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGt                                                  >  1:871124/1‑42 (MQ=255)
                                   aaTTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata                <  1:761557/71‑1 (MQ=255)
                                                  ttAGCCCTGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt  <  1:152202/70‑1 (MQ=255)
                                                  ttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt  <  1:1158867/70‑1 (MQ=255)
                                                  ttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt  <  1:1162262/70‑1 (MQ=255)
                                                  ttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt  <  1:490843/70‑1 (MQ=255)
                                                           |                                                            
TTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT  >  W3110S.gb/1020808‑1020927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑