| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,172,268 | T→C | 100% | K1092E (AAG→GAG) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,172,268 | 0 | T | C | 100.0% | 18.8 / NA | 7 | K1092E (AAG→GAG) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/2); total (5/2) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCC > W3110S.gb/1172237‑1172322 | ccAATCAACCAGGCCGGATTAACTTGATTCTCCTAGGCAAATTCGATCAccc > 1:1775989/1‑52 (MQ=255)ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGa > 1:338559/1‑46 (MQ=255)ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:1687049/1‑71 (MQ=255)ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:204435/1‑71 (MQ=255)ccAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTc > 1:930719/1‑71 (MQ=255) ggATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATccc < 1:564499/71‑1 (MQ=255) ggATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATccc < 1:699904/71‑1 (MQ=255) | CCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCC > W3110S.gb/1172237‑1172322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |