| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,244,320 | C→T | 100% | C113Y (TGC→TAC) | ldcA ← | L,D‑carboxypeptidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,244,320 | 0 | C | T | 100.0% | 33.4 / NA | 12 | C113Y (TGC→TAC) | ldcA | L,D‑carboxypeptidase A |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/6); total (6/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGC > W3110S.gb/1244253‑1244381 | cgcgCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACt > 1:1108148/1‑71 (MQ=255)cgcgCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACt > 1:1292164/1‑71 (MQ=255)cgcgCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACt > 1:523601/1‑71 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:1922637/70‑1 (MQ=255) cTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTaaa < 1:197824/70‑1 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAAt > 1:870974/1‑38 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt > 1:930427/1‑71 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgt > 1:1160086/1‑70 (MQ=255) gcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCg < 1:1570205/71‑1 (MQ=255) gcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCg < 1:1580108/71‑1 (MQ=255) gcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCg < 1:1826903/71‑1 (MQ=255) agaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGc < 1:433299/71‑1 (MQ=255) | CGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGC > W3110S.gb/1244253‑1244381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |